|
| Status |
Public on Feb 05, 2013 |
| Title |
XENO AdVP16hLXRa (LXRa) 8 |
| Sample type |
RNA |
| |
|
| Source name |
Xenograft Tumors (human HT29 cells) grown in Nu/Nu Athymic Female Mice
|
| Organism |
Homo sapiens |
| Characteristics |
cell type (human): Xenograft Tumors (HT29 cells)
strain (mouse): Nu/Nu
age (mouse): 4 months
infection (mouse): LXRa (VP16hLXRa) Adenovirus
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with QIAzol reagent, following manufacturer's instructions. Quality control was performed with Biorad Experion.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion Illumina® TotalPrepTM RNA Amplification Kit
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
XENO AdVP16hLXRa (LXRa) 8
replicate 1
human HT29 cells grown in Nu/Nu mice infected with LXRa (VP16hLXRa) Adenovirus
|
| Data processing |
The data were normalised using quantile normalization with Illumina Genome Studio Software; items with detection p values >0.001 or AVG Signal <100 in both groups were excluded.
|
| |
|
| Submission date |
Feb 05, 2013 |
| Last update date |
Feb 05, 2013 |
| Contact name |
Antonio Moschetta |
| Organization name |
University of Bari & Consorzio Mario Negri Sud
|
| Street address |
Via Nazionale 8A
|
| City |
Santa Maria Imbaro |
| ZIP/Postal code |
66030 |
| Country |
Italy |
| |
|
| Platform ID |
GPL10558 |
| Series (2) |
| GSE44068 |
Genome-wide analysis of gene expression profile of Xenograft Tumors (HT29 cells) grown in Nu/Nu Athymic Female Mice infected with Control (Ad-VP16) or LXRa (VP16hLXRa) Adenovirus |
| GSE44073 |
Liver X Receptors play an antitumoral role in the intestine |
|
