|
| Status |
Public on May 01, 2007 |
| Title |
Cochlea_MC_15mo_2 |
| Sample type |
RNA |
| |
|
| Source name |
cochlea, middle-age control, 15 month-old
|
| Organism |
Mus musculus |
| Characteristics |
Strain: C57BL/6
Gender: male
Age: 15 months old
Tissue: cochlea
|
| Biomaterial provider |
Harlan Sprague-Dawley
|
| Treatment protocol |
To study the effects of CR, we reduced the caloric intake of mice to 74% of that fed to control animals in early adulthood (2 months of age), and this dietary regimen was maintained until 15 months of age. Each control mouse was fed 84 kcal/week of the control diet. Each CR mouse was fed 62 kcal/week of the restricted diet, resulting in a 26% reduction of calorie intake. Mice were divided into three groups: a 4-month-old young control (YC) group, a 15-month-old middle-age control (MC) group, and a 15-month-old calorie-restricted (CR) group. Cochlea tissues were taken from those three groups.
|
| Growth protocol |
All mice were raised under 12:12 dark/light conditions, and housed singly in the specific pathogen-free facility at University of Wisconsin-Madison, and provided a nonpurified diet and acidified water ad libitum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from pooled cochlea tissue using the TriZol Reagent (Invitrogen) according to the manufacturer's instructions.
|
| Label |
Biotin
|
| Label protocol |
cDNA synthesis from total RNA (Invitrogen) and cRNA amplification and labeling (Enzo BioArray IVT kit) were accomplished according to the manufacturer’s instructions (Affymetrix).
|
| |
|
| Hybridization protocol |
Hybridization (hybridization oven 640), and washing and staining (fluidics station 400) were accomplished according to the manufacturer’s instructions (Affymetrix).
|
| Scan protocol |
Arrays were scanned on the GeneChip Scanner 3000 (Affymetrix). Image analysis was performed using GCOS 1.3 (Affymetrix).
|
| Description |
NA
|
| Data processing |
GCOS 1.3 (Affymetrix), scaled to 1500
|
| |
|
| Submission date |
May 06, 2006 |
| Last update date |
Aug 28, 2018 |
| Contact name |
Shinichi Someya |
| E-mail(s) |
someya@wisc.edu
|
| Phone |
608-265-5205
|
| Fax |
608-262-2976
|
| URL |
http://www.genetics.wisc.edu/faculty/profile.php?id=140
|
| Organization name |
University of Wisconsin-Madison
|
| Department |
Medical Genetics
|
| Lab |
Prolla Lab
|
| Street address |
425 Henry Mall Dr
|
| City |
Madison |
| State/province |
WI |
| ZIP/Postal code |
53706 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (1) |
| GSE4786 |
Caloric restriction suppresses apoptotic cell death in the mammalian cochlea and leads to prevention of presbycusis |
|
| Relations |
| Reanalyzed by |
GSE119085 |
