|
Status |
Public on Mar 28, 2014 |
Title |
MEF |
Sample type |
RNA |
|
|
Source name |
MEF
|
Organism |
Mus musculus |
Characteristics |
pluripotent: No background strain: B6D2F1/J
|
Extracted molecule |
total RNA |
Extraction protocol |
500 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 14h)
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
replicate 1
|
Data processing |
The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
|
|
|
Submission date |
Feb 12, 2013 |
Last update date |
Jan 15, 2022 |
Contact name |
Marcos J. Araúzo-Bravo |
E-mail(s) |
mararabra@yahoo.co.uk
|
Phone |
+34 943 00 6108
|
Organization name |
Max Planck Institute for Molecular Biomedicine
|
Department |
Cell and Developmental Biology
|
Lab |
Computational Biology and Bionformatics
|
Street address |
Rogentstrasse
|
City |
Muenster |
ZIP/Postal code |
48149 |
Country |
Germany |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE44284 |
Reprogramming to pluripotency through a somatic stem cell intermediate |
|