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Sample GSM1082315 Query DataSets for GSM1082315
Status Public on Mar 28, 2014
Title iNSC
Sample type RNA
 
Source name iNSC
Organism Mus musculus
Characteristics pluripotent: Yes
background strain: C3H
Extracted molecule total RNA
Extraction protocol 500 ng of total DNA-free RNA was used as input for labelled cRNA synthesis (Illumina TotalPrep RNA Amplification Kit - Ambion) following the manufacturer's instructions (IVT: 14h)
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 1
Data processing The bead intensities were mapped to gene information using BeadStudio 3.2 (Illumina), background correction was perform using the Affymetrix Robust Multi-array Analysis (RMA) background correction model, variance stabilization was performed using the log2 scaling and gene expression normalization was calculated with the quantile method implemented in the lumi package of R-Bioconductor.
 
Submission date Feb 12, 2013
Last update date Mar 28, 2014
Contact name Marcos J Ara├║zo-Bravo
E-mail(s) mararabra@yahoo.co.uk
Organization name Max Planck Institute for Molecular Biomedicine
Department Cell and Developmental Biology
Lab Computational Biology and Bioinformatics
Street address Rontgenstr. 20
City Muenster
ZIP/Postal code 48149
Country Germany
 
Platform ID GPL6885
Series (1)
GSE44284 Reprogramming to pluripotency through a somatic stem cell intermediate

Data table header descriptions
ID_REF
VALUE quantile normalized

Data table
ID_REF VALUE
ILMN_1212607 2.401322
ILMN_1212612 3.977910
ILMN_1212619 5.045605
ILMN_1212628 3.191088
ILMN_1212632 3.528281
ILMN_1212636 11.026553
ILMN_1212637 9.445781
ILMN_1212645 1.477715
ILMN_1212648 7.659621
ILMN_1212653 10.576819
ILMN_1212672 6.449815
ILMN_1212682 2.435527
ILMN_1212683 1.304582
ILMN_1212685 2.448061
ILMN_1212692 1.889267
ILMN_1212693 7.649363
ILMN_1212695 2.042576
ILMN_1212698 1.454501
ILMN_1212702 2.512493
ILMN_1212703 6.726599

Total number of rows: 25697

Table truncated, full table size 553 Kbytes.




Supplementary data files not provided
Processed data included within Sample table
Raw data are available on Series record

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