|
| Status |
Public on Jul 01, 2014 |
| Title |
ce_SRFcko_E8.75_rep3 |
| Sample type |
RNA |
| |
|
| Source name |
ce_SRFcko_E8.75
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57Bl/6
genotype: Srf(-/-)
Stage: embryo
developmental stage: E8.75
tissue: unsegmented caudal end
|
| Treatment protocol |
embryose were dissected in PBS on ice and staged accordingly before tissue preparation.
|
| Growth protocol |
homozygous and heterozygous embryos were obtained by crossing SRF(flex1/+); T:cre/T:cre females to SRF(flex1/+); T:cre/T:cre males
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
replicate 3
|
| Data processing |
The data were normalised using quantile normalisation with Illumina Genome Studio software
|
| |
|
| Submission date |
Feb 19, 2013 |
| Last update date |
Jul 01, 2014 |
| Contact name |
Phillip Grote |
| E-mail(s) |
grote@molgen.mpg.de
|
| Organization name |
Max Planck Institute for Molecular Genetics
|
| Department |
Developmental Genetics
|
| Street address |
Ihnestr. 73
|
| City |
Berlin |
| ZIP/Postal code |
14195 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE44406 |
Srf is essential for mesodermal cell migration during elongation of the embryonic body axis |
|
