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Status |
Public on Jul 01, 2014 |
Title |
ce_wt_E9.0_rep1 |
Sample type |
RNA |
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Source name |
ce_wt_E9.0
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Organism |
Mus musculus |
Characteristics |
strain: C57Bl/6 genotype: Srf(+/-) Stage: embryo developmental stage: E9.0 tissue: unsegmented caudal end
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Treatment protocol |
embryose were dissected in PBS on ice and staged accordingly before tissue preparation.
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Growth protocol |
homozygous and heterozygous embryos were obtained by crossing SRF(flex1/+); T:cre/T:cre females to SRF(flex1/+); T:cre/T:cre males
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Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
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Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
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|
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Hybridization protocol |
Standard Illumina hybridization protocol
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Scan protocol |
Standard Illumina scanning protocol
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Description |
replicate 1
|
Data processing |
The data were normalised using quantile normalisation with Illumina Genome Studio software
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Submission date |
Feb 19, 2013 |
Last update date |
Jul 01, 2014 |
Contact name |
Phillip Grote |
E-mail(s) |
grote@molgen.mpg.de
|
Organization name |
Max Planck Institute for Molecular Genetics
|
Department |
Developmental Genetics
|
Street address |
Ihnestr. 73
|
City |
Berlin |
ZIP/Postal code |
14195 |
Country |
Germany |
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|
Platform ID |
GPL6885 |
Series (1) |
GSE44406 |
Srf is essential for mesodermal cell migration during elongation of the embryonic body axis |
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