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Sample GSM1084601 Query DataSets for GSM1084601
Status Public on Jul 01, 2014
Title ce_wt_E9.0_rep1
Sample type RNA
 
Source name ce_wt_E9.0
Organism Mus musculus
Characteristics strain: C57Bl/6
genotype: Srf(+/-)
Stage: embryo
developmental stage: E9.0
tissue: unsegmented caudal end
Treatment protocol embryose were dissected in PBS on ice and staged accordingly before tissue preparation.
Growth protocol homozygous and heterozygous embryos were obtained by crossing SRF(flex1/+); T:cre/T:cre females to SRF(flex1/+); T:cre/T:cre males
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 1
Data processing The data were normalised using quantile normalisation with Illumina Genome Studio software
 
Submission date Feb 19, 2013
Last update date Jul 01, 2014
Contact name Phillip Grote
E-mail(s) grote@molgen.mpg.de
Organization name Max Planck Institute for Molecular Genetics
Department Developmental Genetics
Street address Ihnestr. 73
City Berlin
ZIP/Postal code 14195
Country Germany
 
Platform ID GPL6885
Series (1)
GSE44406 Srf is essential for mesodermal cell migration during elongation of the embryonic body axis

Data table header descriptions
ID_REF
VALUE quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 3157.279 0
ILMN_2721178 874.6564 0
ILMN_3033922 368.8657 0
ILMN_3092673 731.2416 0
ILMN_2816356 113.676 0
ILMN_2808939 761.7886 0
ILMN_2634564 562.1047 0
ILMN_2737647 61.4694 0.6453634
ILMN_2734484 171.0285 0
ILMN_2952292 103.9078 0
ILMN_2699078 66.39761 0.3421053
ILMN_1213681 327.1683 0
ILMN_2735413 70.30028 0.1528822
ILMN_2735415 73.65343 0.07142857
ILMN_2891688 399.8847 0
ILMN_2637698 712.4711 0
ILMN_2674228 630.1153 0
ILMN_2601546 69.75314 0.1779449
ILMN_1230831 62.95224 0.556391
ILMN_2848071 71.93877 0.1065163

Total number of rows: 25697

Table truncated, full table size 711 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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