|
Status |
Public on Oct 26, 2007 |
Title |
TERA_ATRA_vs_mock_2 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
ATRA-treated TERA2
|
Organism |
Homo sapiens |
Characteristics |
TERA2 human embryonal carcinoma cell line
|
Biomaterial provider |
ATCC
|
Treatment protocol |
2 micromolar all_trans retinoic acid for 12 days
|
Growth protocol |
McCoy's 5A medium with 10% BCS and 1x P/S
|
Extracted molecule |
total RNA |
Extraction protocol |
TriZol extraction followed by RNeasy clean-up with on-column DNase treatment
|
Label |
Cy3
|
Label protocol |
Agilent Low RNA Input Linear Amplification Kit using manufacturer's recommended protocol
|
|
|
Channel 2 |
Source name |
Mock-treated TERA2
|
Organism |
Homo sapiens |
Characteristics |
TERA2 human embryonal carcinoma cell line
|
Biomaterial provider |
ATCC
|
Treatment protocol |
None
|
Growth protocol |
McCoy's 5A medium with 10% BCS and 1x P/S
|
Extracted molecule |
total RNA |
Extraction protocol |
TriZol extraction followed by RNeasy clean-up with on-column DNase treatment
|
Label |
Cy5
|
Label protocol |
Agilent Low RNA Input Linear Amplification Kit using manufacturer's recommended protocol
|
|
|
|
Hybridization protocol |
Agilent Gene Expression Hybridization Kit using manufecturer's recommended protocol
|
Scan protocol |
Microarrays are scanned with Agilent G2565BA microarray scanner under default settings recommended by Agilent Technologies for expression microarrays with 100% PMT and 10 micrometer resolutions. Data are extracted using Feature Extraction Software v8.1 (Agilent Technologies) under default settings for expression microarrays
|
Description |
None
|
Data processing |
log2 Ratio of background subtracted ATRA intensity to background subtracted Mock treated intensity after loess normalization with Bioconductor
|
|
|
Submission date |
May 11, 2006 |
Last update date |
Apr 26, 2007 |
Contact name |
Wayne Yu |
E-mail(s) |
wyu8@jhmi.edu
|
Phone |
(410)502-7970
|
Fax |
(410)955-8780
|
Organization name |
Johns Hopkins University
|
Department |
Cancer Center
|
Street address |
417 N. Caroline St.
|
City |
Baltimore |
State/province |
MD |
ZIP/Postal code |
21231 |
Country |
USA |
|
|
Platform ID |
GPL1708 |
Series (1) |
GSE4809 |
A Stem Cell-Like Chromatin Pattern May Predispose Tumor Suppressor Genes to DNA Hypermethylation |
|