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Sample GSM108548 Query DataSets for GSM108548
Status Public on May 12, 2006
Title Gaido_Testes_pnd2_2_14Dec2005
Sample type RNA
 
Source name testes
Organism Mus musculus
Characteristics gene reported: wild-type
strain: CD-1
Sex: Male
developmental stage: pnd 2
theiler stage: 27
somite count: NA
developmental landmark: NA
Treatment protocol pool size: NA
Pooled sample: No
Dissection Method: Whole organ excision
Experimental Design: CD-1 mice are time-mated within a 2 hour window. Pregnant CD-1 mice are euthanized by standard carbon dioxide asphyxiation. All fetuses are killed by decapitation with a scalpel. Fetal gonads are removed from fetuses at gestation days 11, 12, 14, 16, 18, and postnatal day 2, snap frozen in liquid nitrogen and stored at -80ÂșC.
Extracted molecule total RNA
Extraction protocol Gaido protocol: 'RNA isolation from whole testes'
Label Biotin
Label protocol -
 
Hybridization protocol Affymetrix standard protocol (701021 Rev 4)
Amount labeled target hybridization to array: 10 ug
Scan protocol -
Description >> Amplification protocol <<
Target Amplified manufacturer/kit: NA
Target Amplified protocol: NA
Rounds of amplification: NA
URL: http://gudmap.hgu.mrc.ac.uk/gudmap/pages/mic_submission.html?id=GUDMAP:2169
Data processing Analysis method: Affymetrix GCOS and SAS Microarray Solutions GCOS Tgt value: 500
 
Submission date May 12, 2006
Last update date Aug 28, 2018
Contact name GUDMAP Developers
E-mail(s) gudmap-db@gudmap.org
Phone +44 131 651 8500
Organization name IGMM MRC Human Genetics Unit
Lab GUDMAP Database Group
Street address Crewe Road
City Edinburgh
ZIP/Postal code EH4 2XU
Country United Kingdom
 
Platform ID GPL1261
Series (1)
GSE4818 Gene expression profiling of whole testes in embryonic (gestational day 11, 12, 14, 16 & 18) and postnatal day 2 mice. (GUDMAP Series ID: 3)
Relations
Reanalyzed by GSE119085

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 516.52 P 0.000147
AFFX-BioB-M_at 914.78 P 0.000044
AFFX-BioB-3_at 551.55 P 0.000052
AFFX-BioC-5_at 1737.31 P 0.000044
AFFX-BioC-3_at 2052.56 P 0.000044
AFFX-BioDn-5_at 3531.20 P 0.000044
AFFX-BioDn-3_at 6932.41 P 0.000044
AFFX-CreX-5_at 19675.00 P 0.000044
AFFX-CreX-3_at 23118.71 P 0.000044
AFFX-DapX-5_at 515.05 P 0.000081
AFFX-DapX-M_at 804.78 P 0.000581
AFFX-DapX-3_at 1063.44 P 0.000052
AFFX-LysX-5_at 48.72 P 0.001102
AFFX-LysX-M_at 128.45 P 0.003595
AFFX-LysX-3_at 194.84 P 0.000110
AFFX-PheX-5_at 69.36 P 0.000258
AFFX-PheX-M_at 107.12 P 0.000662
AFFX-PheX-3_at 190.99 P 0.000110
AFFX-ThrX-5_at 144.79 P 0.000195
AFFX-ThrX-M_at 170.32 P 0.000052

Total number of rows: 45101

Table truncated, full table size 1276 Kbytes.




Supplementary file Size Download File type/resource
GSM108548.CEL.gz 3.7 Mb (ftp)(http) CEL

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