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Sample GSM108553 Query DataSets for GSM108553
Status Public on May 12, 2006
Title Gaido_Testes_gd16_7_14Dec2005
Sample type RNA
Source name testes
Organism Mus musculus
Characteristics gene reported: wild-type
strain: CD-1
Sex: Male
developmental stage: gd 16
theiler stage: 24
somite count: NA
developmental landmark: NA
Treatment protocol pool size: NA
Pooled sample: No
Dissection Method: Whole organ excision
Experimental Design: CD-1 mice are time-mated within a 2 hour window. Pregnant CD-1 mice are euthanized by standard carbon dioxide asphyxiation. All fetuses are killed by decapitation with a scalpel. Fetal gonads are removed from fetuses at gestation days 11, 12, 14, 16, 18, and postnatal day 2, snap frozen in liquid nitrogen and stored at -80ÂșC.
Extracted molecule total RNA
Extraction protocol Gaido protocol: 'RNA isolation from whole testes'
Label Biotin
Label protocol -
Hybridization protocol Affymetrix standard protocol (701021 Rev 4)
Amount labeled target hybridization to array: 10 ug
Scan protocol -
Description >> Amplification protocol <<
Target Amplified manufacturer/kit: NA
Target Amplified protocol: NA
Rounds of amplification: NA
Data processing Analysis method: Affymetrix GCOS and SAS Microarray Solutions GCOS Tgt value: 500
Submission date May 12, 2006
Last update date Aug 28, 2018
Contact name GUDMAP Developers
Phone +44 131 651 8500
Organization name IGMM MRC Human Genetics Unit
Lab GUDMAP Database Group
Street address Crewe Road
City Edinburgh
ZIP/Postal code EH4 2XU
Country United Kingdom
Platform ID GPL1261
Series (1)
GSE4818 Gene expression profiling of whole testes in embryonic (gestational day 11, 12, 14, 16 & 18) and postnatal day 2 mice. (GUDMAP Series ID: 3)
Reanalyzed by GSE119085

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
AFFX-BioB-5_at 609.07 P 0.000390
AFFX-BioB-M_at 1252.47 P 0.000044
AFFX-BioB-3_at 755.13 P 0.000052
AFFX-BioC-5_at 2399.22 P 0.000044
AFFX-BioC-3_at 2779.64 P 0.000044
AFFX-BioDn-5_at 4832.62 P 0.000044
AFFX-BioDn-3_at 9177.81 P 0.000044
AFFX-CreX-5_at 24740.80 P 0.000052
AFFX-CreX-3_at 31495.04 P 0.000044
AFFX-DapX-5_at 894.23 P 0.000081
AFFX-DapX-M_at 1245.76 P 0.000446
AFFX-DapX-3_at 1564.60 P 0.000052
AFFX-LysX-5_at 95.97 P 0.001794
AFFX-LysX-M_at 127.91 P 0.001593
AFFX-LysX-3_at 224.51 P 0.000044
AFFX-PheX-5_at 104.95 P 0.000081
AFFX-PheX-M_at 192.50 P 0.000225
AFFX-PheX-3_at 195.01 P 0.000081
AFFX-ThrX-5_at 186.66 P 0.000446
AFFX-ThrX-M_at 263.21 P 0.000052

Total number of rows: 45101

Table truncated, full table size 1278 Kbytes.

Supplementary file Size Download File type/resource
GSM108553.CEL.gz 3.6 Mb (ftp)(http) CEL

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