|
| Status |
Public on Jan 29, 2014 |
| Title |
forebrain_het_male_rep2 |
| Sample type |
RNA |
| |
|
| Source name |
forebrain, male, heterozygote
|
| Organism |
Mus musculus |
| Characteristics |
strain/background: C57BL/6J
genotype: heterozygote
age: E12.5
Sex: male
tissue: forebrain
mouse id: 549_E4
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy midi kits in accordance with the prescribed protocol provided with the kit. Quality control was performed with gel electrophoreses.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol using HiScan.
|
| Description |
Replicate 2
|
| Data processing |
The data were normalised using quantile normalisation with Illumina GenomeStudio 2011.1.
|
| |
|
| Submission date |
Feb 22, 2013 |
| Last update date |
Jan 29, 2014 |
| Contact name |
Martin Irmler |
| Organization name |
Helmholtz Zentrum München GmbH
|
| Department |
Institute of Experimental Genetics
|
| Lab |
Gene Regulation & Epigenetics
|
| Street address |
Ingolstaedter Landstrasse 1
|
| City |
Neuherberg |
| State/province |
Bayern |
| ZIP/Postal code |
85764 |
| Country |
Germany |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE44592 |
Restless Legs Syndrome-associated variant in MEIS1 confers altered gene expression in embryonic ganglionic eminences |
|
