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Sample GSM108821 Query DataSets for GSM108821
Status Public on May 16, 2006
Title H1666(A)
Sample type RNA
 
Source name lung cancer cell line
Organism Homo sapiens
Characteristics Histology: Bronchoalveolar
Age: 5
Sex: F
Race: caucasian
Biomaterial provider Hamon Center for Therapeutic Oncology Research, UT Southwestern Medical Center
Growth protocol RPMI 5% BFS
Extracted molecule total RNA
Extraction protocol Qiagen RNeasy kit including DNase1 treatment
Label biotin
Label protocol Biotinylated cRNA was prepared according to standard Affymetrix protocol from 5 microgram total RNA
 
Hybridization protocol Following fragmentation, 20 microg of cRNA were hybridized for 16 hr at 45C on GeneChip Human Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A
Description None
Data processing Data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 250
 
Submission date May 12, 2006
Last update date Sep 01, 2016
Contact name Luc Girard
E-mail(s) Luc.Girard@UTSouthwestern.edu
Organization name UT Southwestern Medical Center
Department Hamon Center for Therapeutic Oncology Research
Lab NB8.114A
Street address 5323 Harry Hines Blvd
City Dallas
State/province TX
ZIP/Postal code 75390-8593
Country USA
 
Platform ID GPL96
Series (1)
GSE4824 Analysis of lung cancer cell lines
Relations
Reanalyzed by GSE86363

Data table header descriptions
ID_REF Affymetrix IDs
Stat Pairs Number of probe pairs
Stat Pairs Used Number of probe pairs (unmasked)
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)
DETECTION P-VALUE p-value that indicates the significance level of the detection call

Data table
ID_REF Stat Pairs Stat Pairs Used VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 20 20 302.8 P 0.000754
AFFX-BioB-M_at 20 20 380.9 P 0.000081
AFFX-BioB-3_at 20 20 272.8 P 0.000169
AFFX-BioC-5_at 20 20 993.1 P 0.000052
AFFX-BioC-3_at 20 20 1101.0 P 0.000044
AFFX-BioDn-5_at 20 20 1739.5 P 0.000052
AFFX-BioDn-3_at 20 20 4817.8 P 0.000070
AFFX-CreX-5_at 20 20 8738.4 P 0.000044
AFFX-CreX-3_at 20 20 13990.9 P 0.000044
AFFX-DapX-5_at 20 20 16.0 A 0.262809
AFFX-DapX-M_at 20 20 29.0 A 0.147939
AFFX-DapX-3_at 20 20 7.1 A 0.824672
AFFX-LysX-5_at 20 20 13.4 A 0.411380
AFFX-LysX-M_at 20 20 5.3 A 0.699394
AFFX-LysX-3_at 20 20 35.2 A 0.067678
AFFX-PheX-5_at 20 20 10.6 A 0.631562
AFFX-PheX-M_at 20 20 2.3 A 0.904333
AFFX-PheX-3_at 20 20 8.0 A 0.834139
AFFX-ThrX-5_at 20 20 8.3 A 0.631562
AFFX-ThrX-M_at 20 20 22.4 A 0.617401

Total number of rows: 22283

Table truncated, full table size 726 Kbytes.




Supplementary file Size Download File type/resource
GSM108821.CEL.gz 3.3 Mb (ftp)(http) CEL

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