|
| Status |
Public on Dec 31, 2014 |
| Title |
Kidney from lupus NZB/W mouse, rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Kidney from prenephritic mouse, NZB/W, 6 weeks old
|
| Organism |
Mus musculus |
| Characteristics |
strain: NZB/W
age: 6 weeks
disease status: prenephritic, non-proteinuric
tissue: whole kidney
|
| Treatment protocol |
None.
|
| Growth protocol |
None.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Trizol and the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from total RNA (Expression Analysis Technical Manual, Affymetrix).
|
| |
|
| Hybridization protocol |
Biotin labeled cDNA was fragmented and hybridized to Affymetrix Mouse Genome 430 2.0 GeneChip arrays (Santa Clara, CA) according to the manufacturer protocol.
|
| Scan protocol |
GeneChip arrays were scanned by the GeneChip Scanner 3000 7G according to the Affymetrix Expression Analysis Technical Manual.
|
| Description |
Young1_grp1_M1
Gene expression data from NZB/W prenephritic kidney (6 weeks old, non-proteinuric).
|
| Data processing |
The data were analyzed using the GenePattern pipeline: The RMA method and Mouse Entrez Gene custom CDF annotation version 10 were used for the normalization, and Combat for batch correction within the NZB/W mouse model and the NZW/BXSB mouse model.
|
| |
|
| Submission date |
Feb 26, 2013 |
| Last update date |
Dec 31, 2014 |
| Contact name |
Celine C Berthier |
| Organization name |
University of Michigan
|
| Street address |
1150 W. Medical Center Dr, MSRB2
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48109 |
| Country |
USA |
| |
|
| Platform ID |
GPL7546 |
| Series (2) |
| GSE44691 |
Expression data from lupus NZB/W and NZW/BXSB mouse prenephritic kidneys |
| GSE50038 |
Immunopathogenesis of renal inflammation during the progression, remission and relapse of renal disease in the NZB/W murine lupus model |
|
