|
| Status |
Public on Jan 21, 2014 |
| Title |
LW4 |
| Sample type |
RNA |
| |
|
| Source name |
Liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6J
gender: males
age: 29wks
treatment: female mice on low-fat control diet before and during gestation and lactation and male offspring exposed to post-weaning western diet
|
| Treatment protocol |
After 6 wks mice were mated , male offspring where assigned to W or L feeding for 24 wks , generating the 4 exp. Groups WW,WL,LL,LW
|
| Growth protocol |
5 wk old female mouse were randomly assigned to western diet (W) or low fat control diet(L) ,
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with TriReagent ( Sigma). Quality control was performed with Biorad Experion RQI analyser.
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Illumina TotalPrep RNA Amplification Kit ( Applied Biosystems)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
X5511133049_E
L=low-fat control diet , W=western type diet
|
| Data processing |
Batcom ( pmid:16632515) and Quantile normalization with MADMAX pipeline ( pmid : 21778530)
|
| |
|
| Submission date |
Mar 05, 2013 |
| Last update date |
Jan 21, 2014 |
| Contact name |
vincent bloks |
| Organization name |
UMCG
|
| Department |
Pediatrics
|
| Lab |
Pediatrics
|
| Street address |
Hanzeplein1
|
| City |
Groningen |
| ZIP/Postal code |
9713GZ |
| Country |
Netherlands |
| |
|
| Platform ID |
GPL6887 |
| Series (1) |
| GSE44901 |
Maternal western diet primes susceptibility to hepatic inflamation in adult male mouse offspring |
|
