|
Status |
Public on Jun 14, 2013 |
Title |
BMDCs- unstimulated 1 |
Sample type |
RNA |
|
|
Source name |
Mouse bone marrow
|
Organism |
Mus musculus |
Characteristics |
cell type: bone marrow derived Dendritic Cells stimulated: no strain: C57BL/6 Sex: Female time point: 24 hours
|
Treatment protocol |
BMDCs were either treated with media alone or media with 10 ug/mL of antigen lysate from either E. coli or H. pylori.
|
Growth protocol |
BMDCs were generated from the bone marrow of WT female C57BL/6 mice using flt3L at 100 ng/mL for 8 days.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted using Qiagen's Rneasy kit. Quality Control was performed with an Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared using the Illumina TotalPrep RNA Amplification Kit from Ambion.
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol.
|
Scan protocol |
Standard Illumina scanning protocol.
|
Description |
BMDC unstimulated, Biological replicate 1
|
Data processing |
The data were normalized by median method implemented in R package beadarray and log2 transformed.
|
|
|
Submission date |
Mar 07, 2013 |
Last update date |
Jun 14, 2013 |
Contact name |
Jessica Shiu |
E-mail(s) |
jessica.shiu@som.umaryland.edu
|
Organization name |
University of Maryland School of Medicine
|
Department |
Pediatrics
|
Lab |
Blanchard
|
Street address |
655 W Baltimore St
|
City |
Baltimore |
State/province |
MD |
ZIP/Postal code |
21201 |
Country |
USA |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE44954 |
Gene Expression in Bone-marrow derived Dendritic Cells stimulated with H. pylori lysate vs. E. coli lysate |
|