|
| Status |
Public on Jun 14, 2013 |
| Title |
BMDCs- Escherichia coli Ag lysate stimulated 1 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse bone marrow
|
| Organism |
Mus musculus |
| Characteristics |
cell type: bone marrow derived Dendritic Cells
stimulated: Escherichia coli Ag lysate
strain: C57BL/6
Sex: Female
time point: 24 hours
|
| Treatment protocol |
BMDCs were either treated with media alone or media with 10 ug/mL of antigen lysate from either E. coli or H. pylori.
|
| Growth protocol |
BMDCs were generated from the bone marrow of WT female C57BL/6 mice using flt3L at 100 ng/mL for 8 days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using Qiagen's Rneasy kit. Quality Control was performed with an Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared using the Illumina TotalPrep RNA Amplification Kit from Ambion.
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol.
|
| Scan protocol |
Standard Illumina scanning protocol.
|
| Description |
BMDC Escherichia coli stimulated, Biological replicate 1
|
| Data processing |
The data were normalized by median method implemented in R package beadarray and log2 transformed.
|
| |
|
| Submission date |
Mar 07, 2013 |
| Last update date |
Jun 14, 2013 |
| Contact name |
Jessica Shiu |
| E-mail(s) |
jessica.shiu@som.umaryland.edu
|
| Organization name |
University of Maryland School of Medicine
|
| Department |
Pediatrics
|
| Lab |
Blanchard
|
| Street address |
655 W Baltimore St
|
| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21201 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE44954 |
Gene Expression in Bone-marrow derived Dendritic Cells stimulated with H. pylori lysate vs. E. coli lysate |
|
