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Sample GSM1094371 Query DataSets for GSM1094371
Status Public on Jun 14, 2013
Title BMDCs- Escherichia coli Ag lysate stimulated 1
Sample type RNA
 
Source name Mouse bone marrow
Organism Mus musculus
Characteristics cell type: bone marrow derived Dendritic Cells
stimulated: Escherichia coli Ag lysate
strain: C57BL/6
Sex: Female
time point: 24 hours
Treatment protocol BMDCs were either treated with media alone or media with 10 ug/mL of antigen lysate from either E. coli or H. pylori.
Growth protocol BMDCs were generated from the bone marrow of WT female C57BL/6 mice using flt3L at 100 ng/mL for 8 days.
Extracted molecule total RNA
Extraction protocol RNA was extracted using Qiagen's Rneasy kit. Quality Control was performed with an Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared using the Illumina TotalPrep RNA Amplification Kit from Ambion.
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description BMDC Escherichia coli stimulated, Biological replicate 1
Data processing The data were normalized by median method implemented in R package beadarray and log2 transformed.
 
Submission date Mar 07, 2013
Last update date Jun 14, 2013
Contact name Jessica Shiu
E-mail(s) jessica.shiu@som.umaryland.edu
Organization name University of Maryland School of Medicine
Department Pediatrics
Lab Blanchard
Street address 655 W Baltimore St
City Baltimore
State/province MD
ZIP/Postal code 21201
Country USA
 
Platform ID GPL6885
Series (1)
GSE44954 Gene Expression in Bone-marrow derived Dendritic Cells stimulated with H. pylori lysate vs. E. coli lysate

Data table header descriptions
ID_REF
VALUE normalized probe intensity.
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 7.901058415 1
ILMN_3122480 7.115872312 0.43358
ILMN_2599935 8.280383817 1
ILMN_2675543 7.095466101 0.37719
ILMN_2686883 8.295222326 1
ILMN_2751818 7.155838719 0.55138
ILMN_2728634 7.356958342 0.95363
ILMN_3040515 7.175414258 0.61905
ILMN_2711608 7.218511573 0.72932
ILMN_1232875 7.87854362 1
ILMN_1258507 7.764906417 1
ILMN_2746142 7.078930734 0.32331
ILMN_1252690 7.291190572 0.88596
ILMN_2655499 7.074767109 0.30952
ILMN_1252870 7.196644936 0.67293
ILMN_1248179 7.067451725 0.29198
ILMN_2649955 8.347066521 1
ILMN_2628708 7.175414258 0.6203
ILMN_3024781 6.999888441 0.11905
ILMN_2705628 14.63982515 1

Total number of rows: 25697

Table truncated, full table size 764 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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