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Sample GSM1094376 Query DataSets for GSM1094376
Status Public on Jun 14, 2013
Title BMDCs- Helicobacter pylori Ag lysate stimulated 3
Sample type RNA
 
Source name Mouse bone marrow
Organism Mus musculus
Characteristics cell type: bone marrow derived Dendritic Cells
stimulated: Helicobacter pylori Ag lysate
strain: C57BL/6
Sex: Female
time point: 24 hours
Treatment protocol BMDCs were either treated with media alone or media with 10 ug/mL of antigen lysate from either E. coli or H. pylori.
Growth protocol BMDCs were generated from the bone marrow of WT female C57BL/6 mice using flt3L at 100 ng/mL for 8 days.
Extracted molecule total RNA
Extraction protocol RNA was extracted using Qiagen's Rneasy kit. Quality Control was performed with an Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared using the Illumina TotalPrep RNA Amplification Kit from Ambion.
 
Hybridization protocol Standard Illumina hybridization protocol.
Scan protocol Standard Illumina scanning protocol.
Description BMDC Helicobacter pylori stimulated, Biological replicate 3
Data processing The data were normalized by median method implemented in R package beadarray and log2 transformed.
 
Submission date Mar 07, 2013
Last update date Jun 14, 2013
Contact name Jessica Shiu
E-mail(s) jessica.shiu@som.umaryland.edu
Organization name University of Maryland School of Medicine
Department Pediatrics
Lab Blanchard
Street address 655 W Baltimore St
City Baltimore
State/province MD
ZIP/Postal code 21201
Country USA
 
Platform ID GPL6885
Series (1)
GSE44954 Gene Expression in Bone-marrow derived Dendritic Cells stimulated with H. pylori lysate vs. E. coli lysate

Data table header descriptions
ID_REF
VALUE normalized probe intensity.
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_1250052 7.663858715 1
ILMN_3122480 7.158236103 0.68546
ILMN_2599935 8.572396089 1
ILMN_2675543 7.328272497 0.97368
ILMN_2686883 7.758159281 1
ILMN_2751818 7.109291931 0.5
ILMN_2728634 7.227718061 0.88095
ILMN_3040515 7.102013454 0.47619
ILMN_2711608 7.035891688 0.28195
ILMN_1232875 7.595903602 1
ILMN_1258507 7.606229684 1
ILMN_2746142 7.184070176 0.76441
ILMN_1252690 7.297652351 0.95113
ILMN_2655499 7.10721611 0.49373
ILMN_1252870 7.348623638 0.97619
ILMN_1248179 7.033707441 0.27444
ILMN_2649955 7.937096347 1
ILMN_2628708 7.92830476 1
ILMN_3024781 7.035891688 0.27945
ILMN_2705628 14.22450485 1

Total number of rows: 25697

Table truncated, full table size 758 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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