RNA was extracted with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control (RIN>8) was performed with Agilent Bioanalyser.
biotinylated cRNA was prepared using the Illumina TotalPrep RNA Amplification Kit from Applied Biosystems
Standard Illumina hybridization protocol
Standard Illumina scanning protocol
Data was processed using R2.11.1, beadarray 1.16.0. Differential expression was analysed with limma using design matrix design=cbind(M.WT=c(1,0,0,0,1,0,0,0,1,0,0,0,1,0,0,0), M.MD=c(0,1,0,0,0,1,0,0,0,1,0,0,0,1,0,0), F.WT=c(0,0,1,0,0,0,1,0,0,0,1,0,0,0,1,0), F.MD=c(0,0,0,1,0,0,0,1,0,0,0,1,0,0,0,1), Array2=c(0,0,0,0,0,0,0,0,1,1,1,1,1,1,1,1))