|
| Status |
Public on Jul 28, 2006 |
| Title |
heart, patUpDp7 proxT65H, U74B |
| Sample type |
RNA |
| |
|
| Source name |
newborn heart, patUpDp7 proxT65H (=matUpDp11)
|
| Organism |
Mus musculus |
| Characteristics |
Strain: T65H translocation mouse (Beechey et al., Mammalian Genome 8(4):236-240, 1997) having a paternally-derived uniparental duplication of Chr 7 proximal of the T65H breakpoint (7F4) and, consequently, a maternally-derived uniparental duplication of Chr 11 proximal of the T65H breakpoint (11B1.1)
Age: newborn
Tissue: heart
|
| Biomaterial provider |
Colin Beechey, Mammalian Genetics Unit, Harwell, Oxfordshire, UK
|
| Treatment protocol |
Tissues were snap frozen in liquid nitrogen, permanently stored at -80C, and put on dry ice during transport.
|
| Growth protocol |
N/A
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated from tissues in guanidine isothiocyanate by centrifugation through caesium chloride.
|
| Label |
Biotin
|
| Label protocol |
Caesium chloride isolated total RNA was quantified on an Agilent Bioanalyser and 5 to 7µg were used to prepare biotin-labeled cRNA target, as prescribed in the Affymetrix expression manual (Affymetrix, 2004). The biotinylated cRNA target was purified using cRNA Cleanup spin columns (Affymetrix), fragmented with 5x fragmentation buffer (Affymetrix), and quantified prior to hybridization on an Agilent Bioanalyser.
|
| |
|
| Hybridization protocol |
0.15µg of labeled probe was hybridised to the array, washed and stained on the Affymetrix fluidics station 450. Standard protocol.
|
| Scan protocol |
The Affymetrix Scanner 3000 system was used. Standard protocol.
|
| Description |
Tissue-specific comparison of gene expression levels in T65H translocation mice, either with or without uniparental duplications of Chrs 7 & 11. Identification of highly differentially expressed transcripts. See T65H series record.
|
| Data processing |
MAS5/GCOS: TGT=500, otherwise default values.
|
| |
|
| Submission date |
May 19, 2006 |
| Last update date |
Jul 28, 2006 |
| Contact name |
Reiner Schulz |
| E-mail(s) |
reiner.schulz@kcl.ac.uk
|
| Organization name |
King's College London
|
| Department |
Medical & Molecular Genetics
|
| Lab |
Epigenetics
|
| Street address |
8th floor Guy's Tower
|
| City |
London |
| ZIP/Postal code |
SE1 9RT |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL82 |
| Series (1) |
| GSE4870 |
Expression data from T65H translocation mice |
|
