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Sample GSM109821 Query DataSets for GSM109821
Status Public on May 24, 2006
Title endometrium 650
Sample type RNA
 
Source name uterus
Organism Homo sapiens
Characteristics Early secretory phase ESE
Age: 48 Diagnostics: Leiomyomata (TAH) Caucasian
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG 133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner HR3000.
Description Gene expression data from normal human endometrium specimen in proliferative phase.
Data processing The data were analyzed with Gene Chip Operating System GCOS version 1.1 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date May 22, 2006
Last update date Aug 28, 2018
Contact name Said Talbi
E-mail(s) talbis@obgyn.ucsf.edu
Phone 415-514-2846
Organization name UCSF
Street address 500 Parnassus
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
 
Platform ID GPL570
Series (1)
GSE4888 Molecular phenotyping of human endometrium
Relations
Reanalyzed by GSE64985
Reanalyzed by GSE119087

Data table header descriptions
ID_REF Affymetrix ID for HG 133 Plus 2.0 chip
VALUE GCOS-calculated Signal intensity
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE DETECTION P-VALUE
1007_s_at 1032.4364 0.9750803
1053_at 76.96567 0.48200887
117_at 59.017956 0.5712692
121_at 2165.186 0.69386077
1255_g_at 13.813161 0.8536767
1294_at 178.5875 0.96777445
1316_at 89.86107 0.89456075
1320_at 45.65638 0.59103334
1405_i_at 144.08257 0.11639459
1431_at 19.828213 0.7359182
1438_at 361.55725 0.05204102
1487_at 172.13889 0.70752394
1494_f_at 109.82711 0.9872295
1598_g_at 822.9939 0.52144176
160020_at 340.60934 0.3313871
1729_at 272.37534 0.9882936
1773_at 78.98213 0.3826107
177_at 28.361698 0.4738762
179_at 557.3543 0.7634804
1861_at 106.88005 0.93944037

Total number of rows: 54675

Table truncated, full table size 1650 Kbytes.




Supplementary file Size Download File type/resource
GSM109821_650.CEL.gz 8.3 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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