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Sample GSM109825 Query DataSets for GSM109825
Status Public on May 24, 2006
Title endometrium 617
Sample type RNA
 
Source name uterus
Organism Homo sapiens
Characteristics Mid secretory phase MSE
Age: 42 Diagnostics: Pelvic pain (no endo at laparoscopy) TVH/TAH Caucasian
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG 133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner HR3000.
Description Gene expression data from normal human endometrium specimen in proliferative phase.
Data processing The data were analyzed with Gene Chip Operating System GCOS version 1.1 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date May 22, 2006
Last update date Aug 28, 2018
Contact name Said Talbi
E-mail(s) talbis@obgyn.ucsf.edu
Phone 415-514-2846
Organization name UCSF
Street address 500 Parnassus
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
 
Platform ID GPL570
Series (1)
GSE4888 Molecular phenotyping of human endometrium
Relations
Reanalyzed by GSE64985
Reanalyzed by GSE119087

Data table header descriptions
ID_REF Affymetrix ID for HG 133 Plus 2.0 chip
VALUE GCOS-calculated Signal intensity
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE DETECTION P-VALUE
1007_s_at 1237.1946 0.3233896
1053_at 95.82424 0.9856914
117_at 69.09035 0.92052364
121_at 2562.5808 0.59328645
1255_g_at 14.763279 0.7416719
1294_at 179.97504 0.837938
1316_at 95.297585 0.7179382
1320_at 53.521324 0.8556943
1405_i_at 41.315144 0.2566263
1431_at 20.376297 0.8962105
1438_at 128.26273 0.70431036
1487_at 221.49017 0.3637257
1494_f_at 123.00469 0.4484433
1598_g_at 1002.24854 0.91625625
160020_at 400.55334 0.33053938
1729_at 319.35828 0.35277712
1773_at 62.860157 0.86636156
177_at 31.71453 0.6135881
179_at 670.82654 0.22738041
1861_at 101.2884 0.9903009

Total number of rows: 54675

Table truncated, full table size 1650 Kbytes.




Supplementary file Size Download File type/resource
GSM109825_617.CEL.gz 8.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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