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Sample GSM109832 Query DataSets for GSM109832
Status Public on May 24, 2006
Title endometrium 494
Sample type RNA
 
Source name uterus
Organism Homo sapiens
Characteristics Ambiguous histology reading
Age: 45 Diagnostics: Leiomyomata (TAH) Black Norvasc, HCTZ/MSE, uncertainb
Treatment protocol NA
Growth protocol NA
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG 133 Plus 2.0 Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner HR3000.
Description Gene expression data from normal human endometrium specimen in proliferative phase.
Data processing The data were analyzed with Gene Chip Operating System GCOS version 1.1 using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
 
Submission date May 22, 2006
Last update date Aug 28, 2018
Contact name Said Talbi
E-mail(s) talbis@obgyn.ucsf.edu
Phone 415-514-2846
Organization name UCSF
Street address 500 Parnassus
City San Francisco
State/province CA
ZIP/Postal code 94143
Country USA
 
Platform ID GPL570
Series (1)
GSE4888 Molecular phenotyping of human endometrium
Relations
Reanalyzed by GSE64985
Reanalyzed by GSE119087

Data table header descriptions
ID_REF Affymetrix ID for HG 133 Plus 2.0 chip
VALUE GCOS-calculated Signal intensity
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE DETECTION P-VALUE
1007_s_at 822.9107 0.47332525
1053_at 105.235825 0.7780187
117_at 67.18166 0.8324907
121_at 2755.516 0.5397606
1255_g_at 13.980438 0.8800504
1294_at 192.37344 0.75534576
1316_at 79.099724 0.5711351
1320_at 49.93179 0.7533435
1405_i_at 84.88764 0.8281347
1431_at 20.368166 0.78733563
1438_at 98.230385 0.3664034
1487_at 204.61668 0.73539996
1494_f_at 106.94759 0.8918694
1598_g_at 866.3636 0.5626266
160020_at 518.3726 0.5962924
1729_at 285.4197 0.8350648
1773_at 52.045353 0.5167315
177_at 34.096893 0.74336237
179_at 438.2487 0.5518086
1861_at 102.20444 0.9027788

Total number of rows: 54675

Table truncated, full table size 1650 Kbytes.




Supplementary file Size Download File type/resource
GSM109832_494.CEL.gz 8.4 Mb (ftp)(http) CEL
Raw data provided as supplementary file

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