|
| Status |
Public on Jun 20, 2013 |
| Title |
cardiomyocyte untreated rep2 |
| Sample type |
RNA |
| |
|
| Source name |
cardiomyocte untreated
|
| Organism |
Mus musculus |
| Characteristics |
cell line: HL-1
treatment: control
|
| Treatment protocol |
Treated with or without growth factor (10ng/mL TGF-beta2 and 100ng/mL PDGF BB, Invitrogen), incubated in serumfree media for 23h before collection of media.
|
| Growth protocol |
HL-1 cells growth in T75 flasks and keept according to protocol (serum was ultrcentrifuged before use.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was purified with Rneasy Fibrous Tissue Kit (Qiagen)
|
| Label |
Cy3
|
| Label protocol |
Illumina TotalPrep RNA Amplification Kit was used
|
| |
|
| Hybridization protocol |
Performed according to Illumina protocol
|
| Scan protocol |
Illumina Beadstation 500GX according to Illumina protocol
|
| Description |
9253005037_E
|
| Data processing |
Cubic spline normalization, subtracted background.
Illumina Genomestudio was used
|
| |
|
| Submission date |
Mar 15, 2013 |
| Last update date |
Jun 20, 2013 |
| Contact name |
Nina Gennebäck |
| E-mail(s) |
nina.genneback@ncl.ac.uk
|
| Organization name |
Umeå University
|
| Department |
Public health and clinical medicin/medicin
|
| Street address |
NUS 6M
|
| City |
Umeå |
| ZIP/Postal code |
901 86 |
| Country |
Sweden |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE45207 |
Growth factor stimulation of cardiomyocytes induces changes in the transcriptional contents of secreted exosomes |
|
