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Sample GSM1099747 Query DataSets for GSM1099747
Status Public on Jan 13, 2014
Title Blood Sham 2 hr rep 3
Sample type RNA
 
Source name mouse leukocytes
Organism Mus musculus
Characteristics treatment: Sham
genetic background: C57BL/6J
cell type: leukocytes
time: 2 hours
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the manufacturer’s recommended protocol for RNeasy (Qiagen, Valencia, CA).
Label biotin
Label protocol 1-2 μg of total RNA was used to make single-stranded antisense cDNA using the NuGEN Technologies (San Carlo, CA) Ovation™ Biotin System in accordance with the manufacturer’s directions.
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol Labeled targets were hybridized to Affymetrix (Santa Clara, CA) Mouse Genome 430 2.0 Array GeneChip™ microarrays for 16 hours at 45° C. The arrays were washed and scanned according to Affymetrix standard protocols
Description 2 hours after sham LPS
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0/GCOS 1.4 ) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Mar 18, 2013
Last update date Jan 13, 2014
Contact name Wenzhong Xiao, Inflammation & the Host Response to Injury
E-mail(s) data_curator@gluegrant.org
Phone 617 726 0082
Organization name Massachusetts General Hospital
Department Department of Surgery
Lab Burn & Trauma Research
Street address 55 Fruit Street, GRV 1302
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
 
Platform ID GPL1261
Series (1)
GSE7404 Comparison of Longitudinal Leukocyte Gene Expression after Burn Injury or Trauma Hemorrhage in Mice

Data table header descriptions
ID_REF
VALUE MAS 5.0 signal intensity

Data table
ID_REF VALUE
AFFX-BioB-5_at 1049.3
AFFX-BioB-M_at 1531.3
AFFX-BioB-3_at 1521.08
AFFX-BioC-5_at 2231.73
AFFX-BioC-3_at 2098.44
AFFX-BioDn-5_at 4346.25
AFFX-BioDn-3_at 5758.96
AFFX-CreX-5_at 9463.85
AFFX-CreX-3_at 9500.53
AFFX-DapX-5_at 5.84
AFFX-DapX-M_at 19.39
AFFX-DapX-3_at 11.75
AFFX-LysX-5_at 17.13
AFFX-LysX-M_at 20.78
AFFX-LysX-3_at 8.42
AFFX-PheX-5_at 10.37
AFFX-PheX-M_at 11.3
AFFX-PheX-3_at 25.41
AFFX-ThrX-5_at 4.55
AFFX-ThrX-M_at 18.19

Total number of rows: 45101

Table truncated, full table size 765 Kbytes.




Supplementary file Size Download File type/resource
GSM1099747_LPS-SHM_B_2hr_r3.CEL.gz 5.9 Mb (ftp)(http) CEL
Processed data included within Sample table

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