|
Status |
Public on Jan 13, 2014 |
Title |
Blood LPS 1 day rep 3 |
Sample type |
RNA |
|
|
Source name |
mouse leukocytes
|
Organism |
Mus musculus |
Characteristics |
treatment: LPS treated genetic background: C57BL/6J cell type: leukocytes time: 1 day
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using the manufacturer’s recommended protocol for RNeasy (Qiagen, Valencia, CA).
|
Label |
biotin
|
Label protocol |
1-2 μg of total RNA was used to make single-stranded antisense cDNA using the NuGEN Technologies (San Carlo, CA) Ovation™ Biotin System in accordance with the manufacturer’s directions.
|
|
|
Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Drosophila Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
Scan protocol |
Labeled targets were hybridized to Affymetrix (Santa Clara, CA) Mouse Genome 430 2.0 Array GeneChip™ microarrays for 16 hours at 45° C. The arrays were washed and scanned according to Affymetrix standard protocols
|
Description |
1 day after LPS treatment
|
Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0/GCOS 1.4 ) using Affymetrix default analysis settings and global scaling as normalization method.
|
|
|
Submission date |
Mar 18, 2013 |
Last update date |
Jan 13, 2014 |
Contact name |
Wenzhong Xiao, Inflammation & the Host Response to Injury |
E-mail(s) |
data_curator@gluegrant.org
|
Phone |
617 726 0082
|
Organization name |
Massachusetts General Hospital
|
Department |
Department of Surgery
|
Lab |
Burn & Trauma Research
|
Street address |
55 Fruit Street, GRV 1302
|
City |
Boston |
State/province |
MA |
ZIP/Postal code |
02114 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (1) |
GSE7404 |
Comparison of Longitudinal Leukocyte Gene Expression after Burn Injury or Trauma Hemorrhage in Mice |
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