|
Status |
Public on Mar 19, 2013 |
Title |
NF3_ongel_rep2 |
Sample type |
RNA |
|
|
Source name |
Normal mammary fibroblasts, E6 immortalized, cultured on gel 72h
|
Organism |
Mus musculus |
Characteristics |
background strain: FVB/N tumour stage: Normal
|
Treatment protocol |
No treatment
|
Growth protocol |
Cells were isolated from the defined tissues, immortalized using hpvE6 and seeded on a Matrigel:Collagen I deformable matrix and cultured for 72h on 10%FBS DMEM
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
Label |
biotin
|
Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
|
|
Hybridization protocol |
Standard Illumina hybridization protocol
|
Scan protocol |
Standard Illumina scanning protocol
|
Description |
murine fibroblasts from normal mammary gland, E6 immortalized, cultured on Matrigel:collagen matrix for 72h before RNA extraction SAMPLE 4
|
Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
|
|
Submission date |
Mar 18, 2013 |
Last update date |
Mar 19, 2013 |
Contact name |
Fernando Calvo |
E-mail(s) |
fernando.calvo@cancer.org.uk
|
Organization name |
Cancer Research UK
|
Department |
London Research Institute
|
Lab |
Tumour Cell Biology
|
Street address |
44 Lincoln's Inn Fields
|
City |
London |
ZIP/Postal code |
WC2A 3LY |
Country |
United Kingdom |
|
|
Platform ID |
GPL6885 |
Series (1) |
GSE45256 |
Genome-wide analysis of gene expression in murine breast fibroblasts from normal tissue, hyperplasia, adenoma and carcinoma (MMTV-PyMT model) |
|