|
| Status |
Public on Mar 19, 2013 |
| Title |
AdAF1_ongel_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
Adenoma-associated mammary fibroblasts, E6 immortalized, cultured on gel 72h
|
| Organism |
Mus musculus |
| Characteristics |
background strain: FVB/N
tumour stage: Adenoma
|
| Treatment protocol |
No treatment
|
| Growth protocol |
Cells were isolated from the defined tissues, immortalized using hpvE6 and seeded on a Matrigel:Collagen I deformable matrix and cultured for 72h on 10%FBS DMEM
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
murine fibroblasts from mammary adenoma (MMTV-PyMT model), E6 immortalized, cultured on Matrigel:collagen matrix for 72h before RNA extraction
SAMPLE 7
|
| Data processing |
The data were normalised using quantile normalisation with IlluminaGUI in R
|
| |
|
| Submission date |
Mar 18, 2013 |
| Last update date |
Mar 19, 2013 |
| Contact name |
Fernando Calvo |
| E-mail(s) |
fernando.calvo@cancer.org.uk
|
| Organization name |
Cancer Research UK
|
| Department |
London Research Institute
|
| Lab |
Tumour Cell Biology
|
| Street address |
44 Lincoln's Inn Fields
|
| City |
London |
| ZIP/Postal code |
WC2A 3LY |
| Country |
United Kingdom |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE45256 |
Genome-wide analysis of gene expression in murine breast fibroblasts from normal tissue, hyperplasia, adenoma and carcinoma (MMTV-PyMT model) |
|
