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Sample GSM1100470 Query DataSets for GSM1100470
Status Public on Sep 13, 2013
Title NPC_wt_rep2
Sample type RNA
 
Source name Neural progenitor cells
Organism Mus musculus
Characteristics developmental stage: E14.5
genotype: Prmt5F/F
genetic background: mixed C57BL/6 X 129S1/SvlmJ
cell type: NPC
treatment: control
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 2
Data processing The data were normalised using quantile normalisation with beadarray in R
 
Submission date Mar 18, 2013
Last update date Sep 13, 2013
Contact name Julius Müller
Organization name A*Star Singapore
Department IMCB
Lab Ernesto Guccione
Street address 61 Biopolis Drive
City Singapore
ZIP/Postal code 138673
Country Singapore
 
Platform ID GPL6885
Series (2)
GSE45269 Regulation of constitutive and alternative splicing by PRMT5 reveals a role for Mdm4 pre-mRNA in sensing defects in the spliceosomal machinery (BeadChip)
GSE45285 Regulation of constitutive and alternative splicing by PRMT5 reveals a role for Mdm4 pre-mRNA in sensing defects in the spliceosomal machinery

Data table header descriptions
ID_REF
VALUE Quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 3474.2875 0
ILMN_2721178 970.775 0
ILMN_3033922 1541.175 0
ILMN_3092673 5369.3875 0
ILMN_2816356 102.4625 0.02256
ILMN_2808939 2298.7625 0
ILMN_2634564 647.1 0
ILMN_2737647 82.3 0.19674
ILMN_2734484 496.7625 0
ILMN_2952292 205.65 0
ILMN_2699078 67.36875 0.76065
ILMN_1213681 374.175 0
ILMN_2735413 57.5375 0.98246
ILMN_2735415 89.35 0.0802
ILMN_2891688 958.25 0
ILMN_2637698 1147.65 0
ILMN_2674228 578.80625 0
ILMN_2601546 92.7 0.06015
ILMN_1230831 74.6875 0.43609
ILMN_2848071 98.425 0.03383

Total number of rows: 25662

Table truncated, full table size 659 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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