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Sample GSM1100471 Query DataSets for GSM1100471
Status Public on Sep 13, 2013
Title NPC_wt_rep3
Sample type RNA
 
Source name Neural progenitor cells
Organism Mus musculus
Characteristics developmental stage: E14.5
genotype: Prmt5F/F
genetic background: mixed C57BL/6 X 129S1/SvlmJ
cell type: NPC
treatment: control
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 3
Data processing The data were normalised using quantile normalisation with beadarray in R
 
Submission date Mar 18, 2013
Last update date Sep 13, 2013
Contact name Julius Müller
Organization name A*Star Singapore
Department IMCB
Lab Ernesto Guccione
Street address 61 Biopolis Drive
City Singapore
ZIP/Postal code 138673
Country Singapore
 
Platform ID GPL6885
Series (2)
GSE45269 Regulation of constitutive and alternative splicing by PRMT5 reveals a role for Mdm4 pre-mRNA in sensing defects in the spliceosomal machinery (BeadChip)
GSE45285 Regulation of constitutive and alternative splicing by PRMT5 reveals a role for Mdm4 pre-mRNA in sensing defects in the spliceosomal machinery

Data table header descriptions
ID_REF
VALUE Quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 3730.875 0
ILMN_2721178 1051.0375 0
ILMN_3033922 1591.9 0
ILMN_3092673 4929.05 0
ILMN_2816356 103.49375 0.03133
ILMN_2808939 1988.2125 0
ILMN_2634564 742.94375 0
ILMN_2737647 87.575 0.13409
ILMN_2734484 553.7875 0
ILMN_2952292 207.44375 0
ILMN_2699078 69.95 0.64787
ILMN_1213681 441.5 0
ILMN_2735413 86.925 0.1391
ILMN_2735415 78.175 0.32331
ILMN_2891688 1019.20625 0
ILMN_2637698 1180.275 0
ILMN_2674228 617.6125 0
ILMN_2601546 84.0375 0.1817
ILMN_1230831 76.875 0.35338
ILMN_2848071 122.95 0.00125

Total number of rows: 25662

Table truncated, full table size 663 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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