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Sample GSM1100473 Query DataSets for GSM1100473
Status Public on Sep 13, 2013
Title NPC_p5ko_rep1
Sample type RNA
 
Source name Neural progenitor cells
Organism Mus musculus
Characteristics developmental stage: E14.5
genotype: Prmt5F/FNes
genetic background: mixed C57BL/6 X 129S1/SvlmJ
cell type: NPC
treatment: Prmt5 depletion
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 1
Data processing The data were normalised using quantile normalisation with beadarray in R
 
Submission date Mar 18, 2013
Last update date Sep 13, 2013
Contact name Julius Müller
Organization name A*Star Singapore
Department IMCB
Lab Ernesto Guccione
Street address 61 Biopolis Drive
City Singapore
ZIP/Postal code 138673
Country Singapore
 
Platform ID GPL6885
Series (2)
GSE45269 Regulation of constitutive and alternative splicing by PRMT5 reveals a role for Mdm4 pre-mRNA in sensing defects in the spliceosomal machinery (BeadChip)
GSE45285 Regulation of constitutive and alternative splicing by PRMT5 reveals a role for Mdm4 pre-mRNA in sensing defects in the spliceosomal machinery

Data table header descriptions
ID_REF
VALUE Quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 4629.075 0
ILMN_2721178 1257.6875 0
ILMN_3033922 1247.6625 0
ILMN_3092673 3553.7125 0
ILMN_2816356 92.23125 0.06391
ILMN_2808939 1170.8375 0
ILMN_2634564 726.35 0
ILMN_2737647 83.6875 0.1604
ILMN_2734484 594.4625 0
ILMN_2952292 216.4375 0
ILMN_2699078 70.3375 0.61278
ILMN_1213681 305.2 0
ILMN_2735413 77.7125 0.32331
ILMN_2735415 69.575 0.65038
ILMN_2891688 948.0125 0
ILMN_2637698 1787.2375 0
ILMN_2674228 385.05 0
ILMN_2601546 88.25 0.09273
ILMN_1230831 84.3625 0.15038
ILMN_2848071 102.775 0.01504

Total number of rows: 25662

Table truncated, full table size 659 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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