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Sample GSM1100474 Query DataSets for GSM1100474
Status Public on Sep 13, 2013
Title NPC_p5ko_rep2
Sample type RNA
 
Source name Neural progenitor cells
Organism Mus musculus
Characteristics developmental stage: E14.5
genotype: Prmt5F/FNes
genetic background: mixed C57BL/6 X 129S1/SvlmJ
cell type: NPC
treatment: Prmt5 depletion
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 2
Data processing The data were normalised using quantile normalisation with beadarray in R
 
Submission date Mar 18, 2013
Last update date Sep 13, 2013
Contact name Julius Müller
Organization name A*Star Singapore
Department IMCB
Lab Ernesto Guccione
Street address 61 Biopolis Drive
City Singapore
ZIP/Postal code 138673
Country Singapore
 
Platform ID GPL6885
Series (2)
GSE45269 Regulation of constitutive and alternative splicing by PRMT5 reveals a role for Mdm4 pre-mRNA in sensing defects in the spliceosomal machinery (BeadChip)
GSE45285 Regulation of constitutive and alternative splicing by PRMT5 reveals a role for Mdm4 pre-mRNA in sensing defects in the spliceosomal machinery

Data table header descriptions
ID_REF
VALUE Quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 4337.9125 0
ILMN_2721178 1110.675 0
ILMN_3033922 1174.1875 0
ILMN_3092673 4434.4375 0
ILMN_2816356 105.8 0.00501
ILMN_2808939 1196.4 0
ILMN_2634564 591.0625 0
ILMN_2737647 83.4125 0.14411
ILMN_2734484 560.5 0
ILMN_2952292 158.675 0
ILMN_2699078 66.125 0.78947
ILMN_1213681 318.525 0
ILMN_2735413 81.3375 0.18922
ILMN_2735415 81.6125 0.1817
ILMN_2891688 856.675 0
ILMN_2637698 1686.3 0
ILMN_2674228 358.01875 0
ILMN_2601546 81.075 0.19298
ILMN_1230831 75.325 0.36717
ILMN_2848071 95.30625 0.02757

Total number of rows: 25662

Table truncated, full table size 659 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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