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Sample GSM1100475 Query DataSets for GSM1100475
Status Public on Sep 13, 2013
Title NPC_p5ko_rep3
Sample type RNA
 
Source name Neural progenitor cells
Organism Mus musculus
Characteristics developmental stage: E14.5
genotype: Prmt5F/FNes
genetic background: mixed C57BL/6 X 129S1/SvlmJ
cell type: NPC
treatment: Prmt5 depletion
Extracted molecule total RNA
Extraction protocol RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
Label biotin
Label protocol Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
 
Hybridization protocol Standard Illumina hybridization protocol
Scan protocol Standard Illumina scanning protocol
Description replicate 3
Data processing The data were normalised using quantile normalisation with beadarray in R
 
Submission date Mar 18, 2013
Last update date Sep 13, 2013
Contact name Julius Müller
Organization name A*Star Singapore
Department IMCB
Lab Ernesto Guccione
Street address 61 Biopolis Drive
City Singapore
ZIP/Postal code 138673
Country Singapore
 
Platform ID GPL6885
Series (2)
GSE45269 Regulation of constitutive and alternative splicing by PRMT5 reveals a role for Mdm4 pre-mRNA in sensing defects in the spliceosomal machinery (BeadChip)
GSE45285 Regulation of constitutive and alternative splicing by PRMT5 reveals a role for Mdm4 pre-mRNA in sensing defects in the spliceosomal machinery

Data table header descriptions
ID_REF
VALUE Quantile normalized
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2896528 4963.5375 0
ILMN_2721178 1450.3 0
ILMN_3033922 1252.1 0
ILMN_3092673 4481.2375 0
ILMN_2816356 92.74375 0.05514
ILMN_2808939 1386.175 0
ILMN_2634564 592.85625 0
ILMN_2737647 77.1375 0.33083
ILMN_2734484 664.78125 0
ILMN_2952292 219.54375 0
ILMN_2699078 63.3125 0.88471
ILMN_1213681 349.9125 0
ILMN_2735413 75.3375 0.3797
ILMN_2735415 69.95 0.63409
ILMN_2891688 946.9125 0
ILMN_2637698 1555.9875 0
ILMN_2674228 452.4875 0
ILMN_2601546 79.0125 0.26566
ILMN_1230831 91.06875 0.07018
ILMN_2848071 92.5 0.05764

Total number of rows: 25662

Table truncated, full table size 662 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table

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