|
| Status |
Public on May 04, 2014 |
| Title |
DM2 A |
| Sample type |
RNA |
| |
|
| Source name |
Vastus lateralis
|
| Organism |
Homo sapiens |
| Characteristics |
biopsy: DM2 vastus lateralis
age at biopsy: 49
Sex: M
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit. Quality control was performed with Agilent Bioanalyser.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared with the Ambion MessageAmp kit for Illumina arrays
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scanning protocol
|
| Description |
sample A
903
|
| Data processing |
The data was normalised to the median of all samples using quantile normalisation with Inforsense KDE version 2.0.4. Probes were filtered using a unpaired t-test (p=>0.05) using GeneSpring GX version 11.0
|
| |
|
| Submission date |
Mar 20, 2013 |
| Last update date |
May 04, 2014 |
| Contact name |
Mark Andrew Screen |
| E-mail(s) |
mark.screen@helsinki.fi
|
| Organization name |
Folkhälsan Institute of Genetics, University of Helsinki
|
| Department |
Dpt. of Medical Genetics
|
| Lab |
Neuromuscular disorders
|
| Street address |
Biomedicum1, Haartmaninkatu 8
|
| City |
Helsinki |
| ZIP/Postal code |
FI-00290 |
| Country |
Finland |
| |
|
| Platform ID |
GPL6102 |
| Series (1) |
| GSE45331 |
Expression array analysis of myotonic dystrophy type 2 (DM2) muscle biopsies versus controls |
|
