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Sample GSM11030 Query DataSets for GSM11030
Status Public on Oct 09, 2003
Title CVB3_S-0.5h
Sample type RNA
 
Source name HeLa cell lysate
Organism Homo sapiens
Extracted molecule total RNA
 
Description Human cervical carcinoma cells (HeLa cells; American-Type Culture
Collection) were cultured in a 10 cm tissue culture plate. Cells were serum starved overnight and infected with phosphate buffered saline for 45 min under serum-free conditions. Following infection, virus was removed and fresh media containing 10% fetal bovine serum was added for the remainder of the infectious process. At 30 minutes following infection, cells were scraped into 1ml ice cold PBS and spun at 1000 rpm for 10 minutes. Supernatant was removed and cells lysates were flash frozen in liquid nitrogen. Subsequently, RNA was isolated, processed and hybridized to Affymetrix U95A arrays.
 
Submission date Oct 08, 2003
Last update date Mar 16, 2009
Contact name Bruce Maxwell McManus
E-mail(s) Bruce.McManus@hli.ubc.ca
Phone 604-806-8586
Organization name PROOF Centre of Excellence
Department UBC James Hogg Research Centre
Street address 1081 Burrard Street
City Vancouver
State/province BC
ZIP/Postal code V6Z 1Y6
Country Canada
 
Platform ID GPL8300
Series (1)
GSE712 CVB3-infected HeLa cells (multiple time points)

Data table header descriptions
ID_REF
VALUE Raw signal intensity values from Affymetrix MAS 5.0
ABS_CALL Present/Absent/Marginal call as determined in MAS 5.0
DETECTION-p-VALUE p-value that indicates the detection confidence as calculated by the MAS 5.0 software

Data table
ID_REF VALUE ABS_CALL DETECTION-p-VALUE
31307_at 7.8 A 0.968664
31308_at 34.6 A 0.64131
31309_r_at 122.2 A 0.267463
31310_at 40.3 A 0.861235
31311_at 29.3 A 0.985063
31312_at 81.9 A 0.715253
31313_at 33.4 A 0.849473
31314_at 121 A 0.541184
31315_at 88.7 A 0.561639
31316_at 4.4 A 0.999195
31317_r_at 557.6 A 0.127645
31318_at 13.5 A 0.951005
31319_at 54.5 A 0.901946
31320_at 550.7 A 0.418069
31321_at 213.5 A 0.150527
31322_at 58.7 A 0.47938
31323_r_at 95.3 A 0.975289
31324_at 37 A 0.64131
31325_at 39.9 A 0.951005
31326_at 76.8 A 0.861235

Total number of rows: 12558

Table truncated, full table size 315 Kbytes.




Supplementary data files not provided

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