|
Status |
Public on May 01, 2013 |
Title |
WT embryo at E15.5 rep 1 |
Sample type |
RNA |
|
|
Source name |
WT mouse adrenals at E15.5
|
Organism |
Mus musculus |
Characteristics |
strain background: C57BL/6 genotype/variation: WT developmental stage: E15.5 tissue: adrenals
|
Growth protocol |
Sf1-Cre/Dicerlox/lox mice, Sf1-Cre/Dicer+/lox and Dicerlox/lox mice were mated together. Females from each mating pair were monitored for seminal plugs, and the morning of detection was designated as E0.5.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using the RNAqueous Micro kit (Life Technologies, Carlsbad, CA) in accordance with the manufacturer’s protocol.
|
Label |
biotin
|
Label protocol |
standard Affymetrix protocol
|
|
|
Hybridization protocol |
standard Affymetrix protocol
|
Scan protocol |
standard Affymetrix protocol
|
Description |
WT_E15.5-1 Hammer_001R_(Mouse430_2).CEL Gene expression data from WT mouse adrenals at E15.5
|
Data processing |
Microarray data were normalized using the robust multiarray average (RMA) algorithm (limma R package).
|
|
|
Submission date |
Mar 28, 2013 |
Last update date |
May 01, 2013 |
Contact name |
Katherine Gurdziel |
Organization name |
Wayne State University
|
Street address |
275 E. Hancock
|
City |
Detroit |
State/province |
MI |
ZIP/Postal code |
48201 |
Country |
USA |
|
|
Platform ID |
GPL1261 |
Series (2) |
GSE45592 |
Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex [array] |
GSE45812 |
Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex |
|