|
| Status |
Public on May 01, 2013 |
| Title |
KO embryo at E15.5 rep 1 |
| Sample type |
RNA |
| |
|
| Source name |
KO mouse adrenals at E15.5
|
| Organism |
Mus musculus |
| Characteristics |
strain background: C57BL/6
genotype/variation: KO Sf1-Cre/Dicer lox/lox
developmental stage: E15.5
tissue: adrenals
|
| Growth protocol |
Sf1-Cre/Dicerlox/lox mice, Sf1-Cre/Dicer+/lox and Dicerlox/lox mice were mated together. Females from each mating pair were monitored for seminal plugs, and the morning of detection was designated as E0.5.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNAqueous Micro kit (Life Technologies, Carlsbad, CA) in accordance with the manufacturer’s protocol.
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
standard Affymetrix protocol
|
| Scan protocol |
standard Affymetrix protocol
|
| Description |
KO_E15.5-1
Hammer_002R_(Mouse430_2).CEL
Gene expression data from KO mouse adrenals at E15.5
|
| Data processing |
Microarray data were normalized using the robust multiarray average (RMA) algorithm (limma R package).
|
| |
|
| Submission date |
Mar 28, 2013 |
| Last update date |
May 01, 2013 |
| Contact name |
Katherine Gurdziel |
| Organization name |
Wayne State University
|
| Street address |
275 E. Hancock
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48201 |
| Country |
USA |
| |
|
| Platform ID |
GPL1261 |
| Series (2) |
| GSE45592 |
Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex [array] |
| GSE45812 |
Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex |
|
