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Sample GSM1110212 Query DataSets for GSM1110212
Status Public on May 01, 2013
Title KO embryo at E15.5 rep 2
Sample type RNA
 
Source name KO mouse adrenals at E15.5
Organism Mus musculus
Characteristics strain background: C57BL/6
genotype/variation: KO Sf1-Cre/Dicer lox/lox
developmental stage: E15.5
tissue: adrenals
Growth protocol Sf1-Cre/Dicerlox/lox mice, Sf1-Cre/Dicer+/lox and Dicerlox/lox mice were mated together. Females from each mating pair were monitored for seminal plugs, and the morning of detection was designated as E0.5.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the RNAqueous Micro kit (Life Technologies, Carlsbad, CA) in accordance with the manufacturer’s protocol.
Label biotin
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description KO_E15.5-2
Hammer_004_(Mouse430_2).CEL
Gene expression data from KO mouse adrenals at E15.5
Data processing Microarray data were normalized using the robust multiarray average (RMA) algorithm (limma R package).
 
Submission date Mar 28, 2013
Last update date May 01, 2013
Contact name Katherine Gurdziel
Organization name Wayne State University
Street address 275 E. Hancock
City Detroit
State/province MI
ZIP/Postal code 48201
Country USA
 
Platform ID GPL1261
Series (2)
GSE45592 Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex [array]
GSE45812 Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex

Data table header descriptions
ID_REF
VALUE normalized signal intensities

Data table
ID_REF VALUE
1415670_at 9.858359004
1415671_at 10.90001513
1415672_at 11.86878526
1415673_at 8.638286167
1415674_a_at 10.27115089
1415675_at 10.19064071
1415676_a_at 12.46454141
1415677_at 9.131556541
1415678_at 10.84730976
1415679_at 11.65264167
1415680_at 9.424057404
1415681_at 10.64198571
1415682_at 8.310807495
1415683_at 11.39268716
1415684_at 8.398010246
1415685_at 9.636003351
1415686_at 10.06549437
1415687_a_at 11.98397466
1415688_at 10.67687938
1415689_s_at 9.503533628

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM1110212_Hammer_004_Mouse430_2_.CEL.gz 3.5 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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