NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1110216 Query DataSets for GSM1110216
Status Public on May 01, 2013
Title KO embryo at E15.5 rep 4
Sample type RNA
 
Source name KO mouse adrenals at E15.5
Organism Mus musculus
Characteristics strain background: C57BL/6
genotype/variation: KO Sf1-Cre/Dicer lox/lox
developmental stage: E15.5
tissue: adrenals
Growth protocol Sf1-Cre/Dicerlox/lox mice, Sf1-Cre/Dicer+/lox and Dicerlox/lox mice were mated together. Females from each mating pair were monitored for seminal plugs, and the morning of detection was designated as E0.5.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the RNAqueous Micro kit (Life Technologies, Carlsbad, CA) in accordance with the manufacturer’s protocol.
Label biotin
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description KO_E15.5-4
Hammer_008_(Mouse430_2).CEL
Gene expression data from KO mouse adrenals at E15.5
Data processing Microarray data were normalized using the robust multiarray average (RMA) algorithm (limma R package).
 
Submission date Mar 28, 2013
Last update date May 01, 2013
Contact name Katherine Gurdziel
Organization name Wayne State University
Street address 275 E. Hancock
City Detroit
State/province MI
ZIP/Postal code 48201
Country USA
 
Platform ID GPL1261
Series (2)
GSE45592 Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex [array]
GSE45812 Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex

Data table header descriptions
ID_REF
VALUE normalized signal intensities

Data table
ID_REF VALUE
1415670_at 9.631774331
1415671_at 10.73431647
1415672_at 11.97305605
1415673_at 8.738706603
1415674_a_at 10.57524633
1415675_at 10.1416873
1415676_a_at 12.5201418
1415677_at 9.368976222
1415678_at 10.86925108
1415679_at 11.69863293
1415680_at 9.388167961
1415681_at 10.73880775
1415682_at 8.177387931
1415683_at 11.32133231
1415684_at 8.569456905
1415685_at 9.682606312
1415686_at 10.27607203
1415687_a_at 11.45082464
1415688_at 10.85870986
1415689_s_at 9.349000078

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM1110216_Hammer_008_Mouse430_2_.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap