NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM1110220 Query DataSets for GSM1110220
Status Public on May 01, 2013
Title KO embryo at E16.5 rep 2
Sample type RNA
 
Source name KO mouse adrenals at E16.5
Organism Mus musculus
Characteristics strain background: C57BL/6
genotype/variation: KO Sf1-Cre/Dicer lox/lox
developmental stage: E16.5
tissue: adrenals
Growth protocol Sf1-Cre/Dicerlox/lox mice, Sf1-Cre/Dicer+/lox and Dicerlox/lox mice were mated together. Females from each mating pair were monitored for seminal plugs, and the morning of detection was designated as E0.5.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the RNAqueous Micro kit (Life Technologies, Carlsbad, CA) in accordance with the manufacturer’s protocol.
Label biotin
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description KO_E16.5-2
Hammer_012_(Mouse430_2).CEL
Gene expression data from KO mouse adrenals at E16.5
Data processing Microarray data were normalized using the robust multiarray average (RMA) algorithm (limma R package).
 
Submission date Mar 28, 2013
Last update date May 01, 2013
Contact name Katherine Gurdziel
Organization name Wayne State University
Street address 275 E. Hancock
City Detroit
State/province MI
ZIP/Postal code 48201
Country USA
 
Platform ID GPL1261
Series (2)
GSE45592 Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex [array]
GSE45812 Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex

Data table header descriptions
ID_REF
VALUE normalized signal intensities

Data table
ID_REF VALUE
1415670_at 9.320199086
1415671_at 10.81479774
1415672_at 11.78480862
1415673_at 8.494810935
1415674_a_at 10.59730998
1415675_at 10.35949041
1415676_a_at 12.47070907
1415677_at 8.73339194
1415678_at 10.64605071
1415679_at 11.9535878
1415680_at 9.197369862
1415681_at 10.74448652
1415682_at 8.093266914
1415683_at 11.20334459
1415684_at 8.23439851
1415685_at 9.500010435
1415686_at 9.925548751
1415687_a_at 11.5878285
1415688_at 10.71056432
1415689_s_at 8.977724072

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM1110220_Hammer_012_Mouse430_2_.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap