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Sample GSM1110221 Query DataSets for GSM1110221
Status Public on May 01, 2013
Title WT embryo at E16.5 rep 3
Sample type RNA
 
Source name WT mouse adrenals at E16.5
Organism Mus musculus
Characteristics strain background: C57BL/6
genotype/variation: WT
developmental stage: E16.5
tissue: adrenals
Growth protocol Sf1-Cre/Dicerlox/lox mice, Sf1-Cre/Dicer+/lox and Dicerlox/lox mice were mated together. Females from each mating pair were monitored for seminal plugs, and the morning of detection was designated as E0.5.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the RNAqueous Micro kit (Life Technologies, Carlsbad, CA) in accordance with the manufacturer’s protocol.
Label biotin
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description WT_E16.5-3
Hammer_013_(Mouse430_2).CEL
Gene expression data from WT mouse adrenals at E16.5
Data processing Microarray data were normalized using the robust multiarray average (RMA) algorithm (limma R package).
 
Submission date Mar 28, 2013
Last update date May 01, 2013
Contact name Katherine Gurdziel
Organization name Wayne State University
Street address 275 E. Hancock
City Detroit
State/province MI
ZIP/Postal code 48201
Country USA
 
Platform ID GPL1261
Series (2)
GSE45592 Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex [array]
GSE45812 Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex

Data table header descriptions
ID_REF
VALUE normalized signal intensities

Data table
ID_REF VALUE
1415670_at 9.72015216
1415671_at 11.02905573
1415672_at 11.82415584
1415673_at 8.525234864
1415674_a_at 10.06826239
1415675_at 9.629456614
1415676_a_at 12.18841201
1415677_at 9.239195548
1415678_at 10.9300084
1415679_at 11.52097047
1415680_at 9.190309051
1415681_at 10.61022836
1415682_at 8.233994057
1415683_at 11.13741251
1415684_at 8.830404532
1415685_at 9.54185382
1415686_at 10.16951065
1415687_a_at 11.54345405
1415688_at 10.81884005
1415689_s_at 9.786340853

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM1110221_Hammer_013_Mouse430_2_.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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