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Sample GSM1110222 Query DataSets for GSM1110222
Status Public on May 01, 2013
Title KO embryo at E16.5 rep 3
Sample type RNA
 
Source name KO mouse adrenals at E16.5
Organism Mus musculus
Characteristics strain background: C57BL/6
genotype/variation: KO Sf1-Cre/Dicer lox/lox
developmental stage: E16.5
tissue: adrenals
Growth protocol Sf1-Cre/Dicerlox/lox mice, Sf1-Cre/Dicer+/lox and Dicerlox/lox mice were mated together. Females from each mating pair were monitored for seminal plugs, and the morning of detection was designated as E0.5.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the RNAqueous Micro kit (Life Technologies, Carlsbad, CA) in accordance with the manufacturer’s protocol.
Label biotin
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description KO_E16.5-3
Hammer_014_(Mouse430_2).CEL
Gene expression data from KO mouse adrenals at E16.5
Data processing Microarray data were normalized using the robust multiarray average (RMA) algorithm (limma R package).
 
Submission date Mar 28, 2013
Last update date May 01, 2013
Contact name Katherine Gurdziel
Organization name Wayne State University
Street address 275 E. Hancock
City Detroit
State/province MI
ZIP/Postal code 48201
Country USA
 
Platform ID GPL1261
Series (2)
GSE45592 Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex [array]
GSE45812 Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex

Data table header descriptions
ID_REF
VALUE normalized signal intensities

Data table
ID_REF VALUE
1415670_at 9.756396973
1415671_at 10.7441895
1415672_at 11.98417126
1415673_at 8.439354414
1415674_a_at 10.16053048
1415675_at 9.882904769
1415676_a_at 12.24894104
1415677_at 9.152763125
1415678_at 10.82824157
1415679_at 11.53302834
1415680_at 9.521298765
1415681_at 10.62674365
1415682_at 8.193306329
1415683_at 11.21737994
1415684_at 8.580532172
1415685_at 9.478690932
1415686_at 10.20770806
1415687_a_at 11.9016386
1415688_at 10.84021473
1415689_s_at 9.53596612

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM1110222_Hammer_014_Mouse430_2_.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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