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Sample GSM1110224 Query DataSets for GSM1110224
Status Public on May 01, 2013
Title KO embryo at E16.5 rep 4
Sample type RNA
 
Source name KO mouse adrenals at E16.5
Organism Mus musculus
Characteristics strain background: C57BL/6
genotype/variation: KO Sf1-Cre/Dicer lox/lox
developmental stage: E16.5
tissue: adrenals
Growth protocol Sf1-Cre/Dicerlox/lox mice, Sf1-Cre/Dicer+/lox and Dicerlox/lox mice were mated together. Females from each mating pair were monitored for seminal plugs, and the morning of detection was designated as E0.5.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the RNAqueous Micro kit (Life Technologies, Carlsbad, CA) in accordance with the manufacturer’s protocol.
Label biotin
Label protocol standard Affymetrix protocol
 
Hybridization protocol standard Affymetrix protocol
Scan protocol standard Affymetrix protocol
Description KO_E16.5-4
Hammer_016_(Mouse430_2).CEL
Gene expression data from KO mouse adrenals at E16.5
Data processing Microarray data were normalized using the robust multiarray average (RMA) algorithm (limma R package).
 
Submission date Mar 28, 2013
Last update date May 01, 2013
Contact name Katherine Gurdziel
Organization name Wayne State University
Street address 275 E. Hancock
City Detroit
State/province MI
ZIP/Postal code 48201
Country USA
 
Platform ID GPL1261
Series (2)
GSE45592 Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex [array]
GSE45812 Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex

Data table header descriptions
ID_REF
VALUE normalized signal intensities

Data table
ID_REF VALUE
1415670_at 9.885065486
1415671_at 10.89209935
1415672_at 11.97296962
1415673_at 8.451396375
1415674_a_at 9.984498146
1415675_at 9.791041491
1415676_a_at 12.25690758
1415677_at 9.116588095
1415678_at 10.82747868
1415679_at 11.41620634
1415680_at 9.520640859
1415681_at 10.55767066
1415682_at 8.267937828
1415683_at 11.38725847
1415684_at 8.673299639
1415685_at 9.458575501
1415686_at 10.32340519
1415687_a_at 12.18909902
1415688_at 10.81195738
1415689_s_at 9.402326813

Total number of rows: 45101

Table truncated, full table size 1026 Kbytes.




Supplementary file Size Download File type/resource
GSM1110224_Hammer_016_Mouse430_2_.CEL.gz 3.6 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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