|
| Status |
Public on Sep 21, 2016 |
| Title |
IL15RaKO2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse, stellate cell, IL15Ra-knockout,2
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6/129
cell type: stellate cell
genotype/variation: IL15Ra-knockout
|
| Treatment protocol |
Six week-old female IL-15RaKO and wild type (WT) B6/129 mice were purchased from Jackson Laboratories (Bar Harbor, ME). All procedures were performed in accordance with the Institutional Animal Care and Use Committee Protocols of the Mount Sinai School of Medicine. Mouse HSCs were isolated from WT and IL-15RKO mice using an established method with modifications. Briefly, the mouse liver was perfused in situ with pronase and collagenase, followed by Percoll (GE) density gradient centrifugation. HSC cultures were maintained in Dulbecco's Modified Eagle Medium with 20% Fetal Bovine Serum.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was isolated from primary mouse hepatic stellate cell (Passage 0) with Triazol reagent (Invitrogen) followed by a cleanup procedure using QIAGEN RNeasy minicolumns according to manufacturer’s protocol.
|
| Label |
Cy5
|
| Label protocol |
Refer to the Illumina beadarray Assay System Manual
|
| |
|
| Hybridization protocol |
Refer to the Illumina beadarray Assay System Manual
|
| Scan protocol |
Refer to the Illumina beadarray Assay System Manual
|
| Description |
E3-24_2
|
| Data processing |
For each probe, replicated mesurements (beads) were averaged after removing outliers. Data were were then normalized using quantilenormalization by using GenomeStudio software v.3 (Illumina).
|
| |
|
| Submission date |
Mar 28, 2013 |
| Last update date |
Sep 21, 2016 |
| Contact name |
Yujin Hoshida |
| E-mail(s) |
Yujin.Hoshida@UTSouthwestern.edu
|
| Organization name |
University of Texas Southwestern Medical Center
|
| Street address |
5323 Harry Hines Blvd
|
| City |
Dallas |
| State/province |
TX |
| ZIP/Postal code |
75390 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE45612 |
IL-15R-alpha controls hepatic stellate cell fibrogenic potential during murine chronic liver injury |
|
