|
| Status |
Public on Apr 02, 2013 |
| Title |
Vehicle liver rep4 |
| Sample type |
RNA |
| |
|
| Source name |
vehicle_liver
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL6/J
age: 8 weeks
gender: male
treated with: vehicle (PBS) for a week
tissue: liver
|
| Treatment protocol |
The mice were treated with vehicle or S961 (10nMol/week) using Alzet osmotic pump for a week.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with Trizol reagent, followed by clean-up and DNase I treatment with QIAGEN RNeasy mini kit in accordance with the prescribed protocol provided with the kit.
|
| Label |
biotin
|
| Label protocol |
cRNA was labeled with biotin using Illumina TotalPrep RNA Amplification kit (Ambion)
|
| |
|
| Hybridization protocol |
Standard Illumina hybridization protocol
|
| Scan protocol |
Standard Illumina scan protocol
|
| Description |
replicate 4
SAMPLE 4
|
| Data processing |
The data were normalized by average signal on chip with Illumina BeadStudio (v.3.1.3.0).
|
| |
|
| Submission date |
Apr 01, 2013 |
| Last update date |
Apr 02, 2013 |
| Contact name |
Peng Yi |
| E-mail(s) |
pyi@fas.harvard.edu
|
| Organization name |
Harvard University
|
| Department |
SCRB
|
| Lab |
Douglas Melton Lab
|
| Street address |
7 Divinity Ave.
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02138 |
| Country |
USA |
| |
|
| Platform ID |
GPL6885 |
| Series (1) |
| GSE45694 |
Genome-wide analysis of gene expression in liver after insulin receptor antagonist S961 treatment |
|
