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Sample GSM1113589 Query DataSets for GSM1113589
Status Public on Apr 04, 2013
Title mRNA expression in fertilized embryonic stem cells, rep 1
Sample type RNA
 
Source name Mouse fertilized embryonic stem cell (fESC)
Organism Mus musculus
Characteristics strain: B6129F1
gender: female and male
cell type: fertilized embryonic stem cells
chromosomes contained: both X and Y
passage: 6
Extracted molecule total RNA
Extraction protocol Total RNA extraction was performed with the mirVana extraction kit (Ambion).
Label Biotin
Label protocol Biotinylated cRNA were prepared from 0.55 ug total RNA using the Illumina TotalPrep RNA Amplification Kit (Ambion, Austin,TX).
 
Hybridization protocol Following fragmentation, 0.75 ug of cRNA were hybridized to the Illumina MouseRef-8 Expression BeadChip according to the protocols provided by the manufacturer.
Scan protocol Arrays were scanned using the Illumina Bead Array Reader Confocal Scanner.
Description Sample 6
Fertilized embryonic stem cell (fESC, FE).
Data processing Array data export processing and analysis was performed using Illumina BeadStudio v3.1.3 (Gene Expression Module v3.3.8). Array data were filtered by detection p-value < 0.05 (similar to signal to noise) in at least 50% samples (we applied a filtering criterion for data analysis; higher signal value was required to obtain a detection p-value < 0.05). Selected gene signal values were transformed by logarithm and normalized by the quantile method.
 
Submission date Apr 03, 2013
Last update date Apr 04, 2013
Contact name Nam-Hyung Kim
E-mail(s) nhkim@chungbuk.ac.kr
Organization name Chungbuk National University
Street address Gaeshin-dong
City Cheongju
State/province Chungbuk
ZIP/Postal code 361-763
Country South Korea
 
Platform ID GPL6885
Series (2)
GSE45749 mRNA expression profiles in androgenetic, parthenogenetic and fertilized embryonic stem cells
GSE45754 mRNA and microRNA expression profiles in androgenetic, parthenogenetic and fertilized embryonic stem cells

Data table header descriptions
ID_REF
VALUE Log2-transformed quantile-normalized signal intensity
Detection Pval

Data table
ID_REF VALUE Detection Pval
ILMN_2470407 9.972824 0
ILMN_2491565 10.54138 0
ILMN_2741063 9.605413 0
ILMN_1217063 11.443562 0
ILMN_2693246 7.868849 0.002506266
ILMN_2746107 9.154789 0
ILMN_2680054 11.379888 0
ILMN_1232038 9.752544 0
ILMN_2751804 11.64573 0
ILMN_2757844 11.668366 0
ILMN_2630018 11.154045 0
ILMN_1226157 7.8064575 0.003759399
ILMN_3155363 7.771846 0.005012531
ILMN_1221274 7.7124496 0.005012531
ILMN_3111877 11.038532 0
ILMN_2831283 10.347376 0
ILMN_2793522 7.5856304 0.006265664
ILMN_2617468 11.30558 0
ILMN_2654997 7.7095656 0.005012531
ILMN_1259368 9.030139 0

Total number of rows: 12891

Table truncated, full table size 345 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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