|
| Status |
Public on Jul 07, 2006 |
| Title |
b NOD-scid female, 15 week submandibular gland, replicate 2 |
| Sample type |
RNA |
| |
|
| Source name |
15 week old NOD-scid mouse #2 submandibular glands
|
| Organism |
Mus musculus |
| Characteristics |
Strain: NOD.CB17-Prkdc scid/J
Age: 15 weeks
Gender: female
|
| Treatment protocol |
Submandibular glands were separated away from sublingual glands and connective and adipose tissue of the salivary glands before homogenization in Trizol reagent (Invitrogen).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 microg of cRNA were hybridized on a Murine Genome U74Av2 microarray per the manufacturer instructions (Affymetrix) without additional amplification steps.
|
| Scan protocol |
GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
|
| Description |
NOD-scid female, 15 week submandibular gland, replicate 2
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The mean target intensity of each array was arbitrarily set to 2500.
|
| |
|
| Submission date |
May 30, 2006 |
| Last update date |
Jul 24, 2015 |
| Contact name |
Rodolfo Chaparro |
| Organization name |
Albert Einstein College of Medicine
|
| Department |
Microbiology and Immunology
|
| Lab |
Golding Building, Room 105
|
| Street address |
1300 Morris Park Avenue
|
| City |
Bronx |
| State/province |
NY |
| ZIP/Postal code |
10461 |
| Country |
USA |
| |
|
| Platform ID |
GPL82 |
| Series (1) |
| GSE4953 |
Expression data from NOD-scid vs B6-scid pancreas, submandibular glands, & lacrimal glands |
|
