|
| Status |
Public on Apr 04, 2013 |
| Title |
miRNA expression in fertilized embryonic stem cells, rep 2 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse fertilized embryonic stem cell (fESC)
|
| Organism |
Mus musculus |
| Characteristics |
strain: B6129F1
gender: female and male
cell type: fertilized embryonic stem cells
chromosomes contained: both X and Y
passage: 7
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA extraction was performed with the mirVana extraction kit (Ambion).
|
| Label |
Biotin
|
| Label protocol |
Biotinylated cDNA were prepared from 0.2-1ug total RNA. The miRNA method targets specific sequences with sets of oligonucleotides that are extended and labeled during PCR amplification.
|
| |
|
| Hybridization protocol |
Fluorescently labelled cDNA PCR products were hybridized to the Illumina Mouse v2 MicroRNA expression BeadChip according to the protocols provided by the manufacturer.
|
| Scan protocol |
Arrays were scanned using the Illumina Bead Array Reader Confocal Scanner.
|
| Description |
Sample 7
small RNA
Fertilized embryonic stem cell (fESC, FE).
|
| Data processing |
Array data export processing and analysis was performed using Illumina BeadStudio v3.1.3 (Gene Expression Module v3.3.8). Array data were filtered by detection p-value < 0.05 (similar to signal to noise) in at least 50% samples (we applied a filtering criterion for data analysis; higher signal value was required to obtain a detection p-value < 0.05). Selected gene signal values were transformed by logarithm and normalized by the quantile method.
|
| |
|
| Submission date |
Apr 03, 2013 |
| Last update date |
Apr 04, 2013 |
| Contact name |
Nam-Hyung Kim |
| E-mail(s) |
nhkim@chungbuk.ac.kr
|
| Organization name |
Chungbuk National University
|
| Street address |
Gaeshin-dong
|
| City |
Cheongju |
| State/province |
Chungbuk |
| ZIP/Postal code |
361-763 |
| Country |
South Korea |
| |
|
| Platform ID |
GPL8181 |
| Series (2) |
| GSE45753 |
microRNA expression profiles in androgenetic, parthenogenetically activated and fertilized embryonic stem cells |
| GSE45754 |
mRNA and microRNA expression profiles in androgenetic, parthenogenetic and fertilized embryonic stem cells |
|
