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Sample GSM111381 Query DataSets for GSM111381
Status Public on Jul 07, 2006
Title c NOD-scid female, 6 week pancreas, replicate 5
Sample type RNA
 
Source name 6 week old NOD-scid mouse #5 pancreas
Organism Mus musculus
Characteristics Strain: NOD.CB17-Prkdc scid/J
Age: 6 weeks
Gender: female
Treatment protocol pancreas was removed and immediately homogenized in Trizol reagent (Invitrogen).
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized on a Murine Genome U74Av2 microarray per the manufacturer instructions (Affymetrix) without additional amplification steps.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description NOD-scid female, 6 week pancreas, replicate 5
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The mean target intensity of each array was arbitrarily set to 2500.
 
Submission date May 30, 2006
Last update date Jul 24, 2015
Contact name Rodolfo Chaparro
Organization name Albert Einstein College of Medicine
Department Microbiology and Immunology
Lab Golding Building, Room 105
Street address 1300 Morris Park Avenue
City Bronx
State/province NY
ZIP/Postal code 10461
Country USA
 
Platform ID GPL83
Series (1)
GSE4953 Expression data from NOD-scid vs B6-scid pancreas, submandibular glands, & lacrimal glands

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 2297.3 A 0.470241
AFFX-MurIL10_at 3646 A 0.39692
AFFX-MurIL4_at 825 A 0.382599
AFFX-MurFAS_at 2483.1 A 0.216524
AFFX-BioB-5_at 17411.7 P 0.00762
AFFX-BioB-M_at 27009.2 P 0.000195
AFFX-BioB-3_at 14557.4 P 0.000857
AFFX-BioC-5_at 37697 P 0.000147
AFFX-BioC-3_at 29907.4 P 0.000127
AFFX-BioDn-5_at 21908.9 P 0.00006
AFFX-BioDn-3_at 195008.1 P 0.000258
AFFX-CreX-5_at 421816.9 P 0.000044
AFFX-CreX-3_at 581468.4 P 0.000044
AFFX-BioB-5_st 471.2 A 0.645547
AFFX-BioB-M_st 3586.3 A 0.382599
AFFX-BioB-3_st 1131.1 A 0.645547
AFFX-BioC-5_st 662.1 A 0.904333
AFFX-BioC-3_st 343.6 A 0.804734
AFFX-BioDn-5_st 12595.2 A 0.147939
AFFX-BioDn-3_st 11467.6 P 0.015183

Total number of rows: 11934

Table truncated, full table size 325 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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