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Sample GSM111385 Query DataSets for GSM111385
Status Public on Jul 07, 2006
Title c C57BL/6-scid female, 9 week submandibular gland, replicate 4
Sample type RNA
 
Source name 9 week old B6-scid mouse #4 submandibular glands
Organism Mus musculus
Characteristics Strain: B6.CB17-Prkdc scid/SzJ
Age: 9 weeks
Gender: female
Treatment protocol Submandibular glands were separated away from sublingual glands and connective and adipose tissue of the salivary glands before homogenization in Trizol reagent (Invitrogen).
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 10 microg total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 microg of cRNA were hybridized on a Murine Genome U74Av2 microarray per the manufacturer instructions (Affymetrix) without additional amplification steps.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A.
Description C57BL/6-scid female, 9 week submandibular gland, replicate 4
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The mean target intensity of each array was arbitrarily set to 2500.
 
Submission date May 30, 2006
Last update date Jul 24, 2015
Contact name Rodolfo Chaparro
Organization name Albert Einstein College of Medicine
Department Microbiology and Immunology
Lab Golding Building, Room 105
Street address 1300 Morris Park Avenue
City Bronx
State/province NY
ZIP/Postal code 10461
Country USA
 
Platform ID GPL83
Series (1)
GSE4953 Expression data from NOD-scid vs B6-scid pancreas, submandibular glands, & lacrimal glands

Data table header descriptions
ID_REF
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE 'detection p-value', p-value that indicates the significance level of the detection call

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-MurIL2_at 947.1 A 0.368438
AFFX-MurIL10_at 903.8 A 0.631562
AFFX-MurIL4_at 1236.9 A 0.645547
AFFX-MurFAS_at 2904.3 A 0.440646
AFFX-BioB-5_at 12181.4 P 0.039661
AFFX-BioB-M_at 18270.8 P 0.001796
AFFX-BioB-3_at 6870.2 P 0.036569
AFFX-BioC-5_at 25040.3 P 0.00007
AFFX-BioC-3_at 33887.7 P 0.000081
AFFX-BioDn-5_at 45447.4 P 0.000044
AFFX-BioDn-3_at 274772.5 P 0.000044
AFFX-CreX-5_at 343485.2 P 0.000044
AFFX-CreX-3_at 658800.7 P 0.000044
AFFX-BioB-5_st 5319.2 A 0.131361
AFFX-BioB-M_st 10868.3 A 0.095667
AFFX-BioB-3_st 7313.1 A 0.659339
AFFX-BioC-5_st 1522.6 A 0.724854
AFFX-BioC-3_st 951.2 A 0.58862
AFFX-BioDn-5_st 4266.3 A 0.287743
AFFX-BioDn-3_st 7530 P 0.026111

Total number of rows: 11934

Table truncated, full table size 325 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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