|
Status |
Public on May 01, 2013 |
Title |
3) e15.5 WT |
Sample type |
RNA |
|
|
Source name |
WT mouse adrenals at E15.5
|
Organism |
Mus musculus |
Characteristics |
strain: C57BL/6 tissue: adrenals genotype: WT age: E15.5
|
Growth protocol |
Sf1-Cre/Dicerlox/lox mice, Sf1-Cre/Dicer+/lox and Dicerlox/lox mice were mated together. Females from each mating pair were monitored for seminal plugs, and the morning of detection was designated as E0.5.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using the RNAqueous Micro kit (Life Technologies, Carlsbad, CA) in accordance with the manufacturer’s protocol.
|
Label |
FAM
|
Label protocol |
Megaplex ™ RT Primers were used to label the cDNA. Protocol specified in TaqMan(R) OpenArray(R) MicroRNA Panels User Guide June 2011.
|
|
|
Hybridization protocol |
n/a
|
Scan protocol |
n/a
|
Description |
WT_E15.5-2 microRNA data from WT mouse adrenals at E15.5
|
Data processing |
The non-normalized_data.txt file contains the preprocessed data (CT values) exported from the OpenArray ® Real-Time qPCR Analysis Software. Data were analyzed with the Applied Biosystems DataAssist ™ Software. CT values normalized to U6 using the Applied Biosystems DataAssist ™ Software. Fold change (KO/WT)
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|
|
Submission date |
Apr 05, 2013 |
Last update date |
May 01, 2013 |
Contact name |
Katherine Gurdziel |
Organization name |
Wayne State University
|
Street address |
275 E. Hancock
|
City |
Detroit |
State/province |
MI |
ZIP/Postal code |
48201 |
Country |
USA |
|
|
Platform ID |
GPL16971 |
Series (2) |
GSE45810 |
Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex [RT-PCR] |
GSE45812 |
Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex |
|