|
| Status |
Public on May 01, 2013 |
| Title |
12) e16.5 KO |
| Sample type |
RNA |
| |
|
| Source name |
KO mouse adrenals at E16.5
|
| Organism |
Mus musculus |
| Characteristics |
strain: C57BL/6
tissue: adrenals
genotype: KO Sf1-Cre/Dicer lox/lox
age: E16.5
|
| Growth protocol |
Sf1-Cre/Dicerlox/lox mice, Sf1-Cre/Dicer+/lox and Dicerlox/lox mice were mated together. Females from each mating pair were monitored for seminal plugs, and the morning of detection was designated as E0.5.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using the RNAqueous Micro kit (Life Technologies, Carlsbad, CA) in accordance with the manufacturer’s protocol.
|
| Label |
FAM
|
| Label protocol |
Megaplex ™ RT Primers were used to label the cDNA. Protocol specified in TaqMan(R) OpenArray(R) MicroRNA Panels User Guide June 2011.
|
| |
|
| Hybridization protocol |
n/a
|
| Scan protocol |
n/a
|
| Description |
KO_E16.5-2
microRNA data from KO mouse adrenals at E16.5
|
| Data processing |
The non-normalized_data.txt file contains the preprocessed data (CT values) exported from the OpenArray ® Real-Time qPCR Analysis Software. Data were analyzed with the Applied Biosystems DataAssist ™ Software.
CT values normalized to U6 using the Applied Biosystems DataAssist ™ Software.
Fold change (KO/WT)
|
| |
|
| Submission date |
Apr 05, 2013 |
| Last update date |
May 01, 2013 |
| Contact name |
Katherine Gurdziel |
| Organization name |
Wayne State University
|
| Street address |
275 E. Hancock
|
| City |
Detroit |
| State/province |
MI |
| ZIP/Postal code |
48201 |
| Country |
USA |
| |
|
| Platform ID |
GPL16971 |
| Series (2) |
| GSE45810 |
Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex [RT-PCR] |
| GSE45812 |
Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex |
|
