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Sample GSM1115893 Query DataSets for GSM1115893
Status Public on May 01, 2013
Title 12) e16.5 KO
Sample type RNA
 
Source name KO mouse adrenals at E16.5
Organism Mus musculus
Characteristics strain: C57BL/6
tissue: adrenals
genotype: KO Sf1-Cre/Dicer lox/lox
age: E16.5
Growth protocol Sf1-Cre/Dicerlox/lox mice, Sf1-Cre/Dicer+/lox and Dicerlox/lox mice were mated together. Females from each mating pair were monitored for seminal plugs, and the morning of detection was designated as E0.5.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using the RNAqueous Micro kit (Life Technologies, Carlsbad, CA) in accordance with the manufacturer’s protocol.
Label FAM
Label protocol Megaplex ™ RT Primers were used to label the cDNA. Protocol specified in TaqMan(R) OpenArray(R) MicroRNA Panels User Guide June 2011.
 
Hybridization protocol n/a
Scan protocol n/a
Description KO_E16.5-2
microRNA data from KO mouse adrenals at E16.5
Data processing The non-normalized_data.txt file contains the preprocessed data (CT values) exported from the OpenArray ® Real-Time qPCR Analysis Software. Data were analyzed with the Applied Biosystems DataAssist ™ Software.
CT values normalized to U6 using the Applied Biosystems DataAssist ™ Software.
Fold change (KO/WT)
 
Submission date Apr 05, 2013
Last update date May 01, 2013
Contact name Katherine Gurdziel
Organization name Wayne State University
Street address 275 E. Hancock
City Detroit
State/province MI
ZIP/Postal code 48201
Country USA
 
Platform ID GPL16971
Series (2)
GSE45810 Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex [RT-PCR]
GSE45812 Dicer deficiency reveals microRNAs predicted to control gene expression in developing adrenal cortex

Data table header descriptions
ID_REF
VALUE CT values normalized to U6

Data table
ID_REF VALUE
ath-miR159a null
dme-miR-7 8.981
hsa-let-7a 7.338
hsa-let-7b 5.903
hsa-let-7b# null
hsa-let-7c 5.124
hsa-let-7d 7.896
hsa-let-7e 5.343
hsa-let-7e# 22.86
hsa-let-7f 10.939
hsa-let-7f-1# null
hsa-let-7g 8.66
hsa-let-7i 7.157
hsa-let-7i# null
hsa-miR-1 13.589
hsa-miR-100 6.449
hsa-miR-101 13.523
hsa-miR-103 9.293
hsa-miR-106b 8.509
hsa-miR-106b# 9.725

Total number of rows: 747

Table truncated, full table size 13 Kbytes.




Supplementary data files not provided
Raw data are available on Series record
Processed data included within Sample table
Processed data are available on Series record

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